Isolation and molecular characterization of Escherichia coli O157 H7 and other pathogenic E coli from Dairy herds feaces and wastewater of Solan District of Himachal Pradesh

Abstract

newline newline ABSTRACT newline Escherichia coli is the predominant facultative anaerobe of the human colonic flora. The organism typically colonizes the infant gastrointestinal tract within hours of life. In present study, 50 feacal samples were collected from various herd s at different places in and around Solan, thus all collected samples were processed for isolation and biochemical identification. Out of 50, 36 (72%) found positive for E.coli as discussed under Table(4.2). All isolates were antigenically characterised for O antigen Table(4.3) and among 28, 8 (22%) isolates were untypable, 5 (13.8%) were O153, 5(13.8%) were O11, 3(8.3%) O60, 3(8.3%) O90, 3(8.3%) O91, 2(5.5%) O147, 2(5.5%) O4, 2(5.5%) O6, 1(2.7%) O35, 1(2.7%) O135, 1(2.7%) O106; All isolates were subjected to polymerase chain reaction for the presence of stx1,stx2, eaeA, hlyA genes among strains of E.coli isolated from Dairy Herd s feaces. Among 36 samples 4(11%) samples were found positive for the presence of eaeA gene while on among all 1(2.7%) found positive for stx2 gene. newline