Polymorphic regions in the interleukin 1 fc gamma receptors tnfa genes and susceptibility to chronic periodontitis a genetic association study

Abstract

The present study evaluated the association between the SNP s in IL1 A (-889, +4845), IL1B 511 3954 and FCGR2A H131R FCGR2B Ile 232Thre TNFA 1031 863 and periodontitis susceptibility in a hospital based cohort from South India. In addition a co-relation of the gingival crevicular fluid levels of Interleukin beta and Tumor necrosis factor alpha with the SNP genotype IL1B 511 3954 TNFA 1031 863 was also performed to assess the functionality of the SNP. Linkage disequilibrium analysis and Mult factorial dimensionality reduction analysis was also performed to identify the presence of any interaction between SNP of the genes evaluated A combination of Sanger sequencing and Real time SNP genotyping methods was used to identify the genotypes and cytometric bead array for the protein assessment in gingival crevicular fluid. A significantly higher distribution of the variant alleles in IL1B 3954 IL1RN FCGR2A H131R was observed amongst subjects with chronic periodontitis. An increase in risk ranging from 175 19 was observed when the subjects carried the variant alleles of the IL1B 3954 IL1RN, FCGR2A H131R newline