Isolation, chaeacterization & Study of Microorganism from post operatively infected Patients in Hospitals

Abstract

Antibiotic discovery has open a new era in the treatment of post operative wound newlineinfection. Soon after the emergence of antibiotics, resistant strains were observed. Later newlinehalf century has resulted an alarming increase in this phenomenon. In the present newlinestudy, total 5810 cases of both gender and different age groups, selected from different newlinegeneral surgery ward were studied for the period from July 2008 to December 2011 (the newline Study Period ). The patients were operated for various surgical procedures by different newlinesurgeons. All types of surgeries are included in study group whether emergency or newlineelective, whether performed in main operation theatre or minor operation theatre, clean, newlinepotentially contaminated or even frankly infected cases were also included. Our study newlinehas justified the above statement. Our entire research work was carried out on the newlineemergence of resistant strains in post operative wound infection scanning, identification newlineand anti microbial susceptibility testing. The complete study was carried out on newlineMicroScan autoSCAN4 (USA-FDA approved system) for organism identification to the newlinespecies level and determining antimicrobial agent susceptibility in minimum inhibitory newlineconcentration (MIC) value. The post operative wound infection rate observed in study is newline09.79%. The isolated microbial strains include Staphylococcus Spp., Enterococcus newlineSpp., E. coli, Klebsiella Spp. Pseudomonas Spp. Proteus Spp., Candida Spp. and many newlineothers. Many of them are Methicillin Resistant Staphylococcus aureus, Extended newlineSpectrum Beta-Lactamase producers and KPC carbapenemase producer strains. All of newlinethem were analyzed for antimicrobial susceptibility using around 30 routinely used newlineantibiotics for treatment of infection. The alarming speed of acquiring resistance against newlineantibiotics now suggests the proper usage of antibiotics.