Phytochemical investigation and pharmacological screening of Cedrus deodara Loud and Pinus roxburghii Sarg for memory enhancing wound healing and antiulcer activities

Abstract

newline The research work highlights the pharmacological screening of Cedrus deodara Loud. and Pinus roxburghii Sarg. for memory enhancing, wound healing and antiulcer activities and to isolate the compounds from most active sample by bioactivity guided parting. The stem woods of selected plants were collected from naturally growing regions of Pauri Garhwal, Uttarakhand, India and authenticated. The plant materials were dried, powdered and subjected to steam distillation by using Clevenger apparatus to isolate volatile oils and also extracted successively with chloroform and methanol using Soxhlet apparatus to obtain extracts. newline The constituents present in the volatile oils and extracts were screened by phytochemical testing and thin layer chromatography profiling. The preliminary phytochemical investigation of volatile oils of Cedrus deodara Loud. showed the presence of terpenoids, steroids, phenolic compounds, fixed oils and fats, whereas volatile oils of Pinus roxburghii Sarg. showed the presence of alkaloids, glycosides, terpenoids, steroids, fixed oils and fats. The chloroform extract of Cedrus deodara Loud. showed the presence of alkaloids, flavonoids, steroids, terpenoids, tannins, glycosides, fixed oils and carbohydrates, whereas methanolic extract contained alkaloids, flavonoids, steroids, terpenoids, tannins, glycosides and carbohydrates. Moreover, chloroform and methanolic extracts of Pinus roxburghii Sarg. contained alkaloids, glycosides, flavonoids, terpenoids, saponins, tannins and carbohydrates. TLC profiling of volatile oils and chloroform extracts showed utmost number of spots on plates than methanolic extracts. These results revealed that oils and chloroform extracts of Cedrus deodara Loud. and Pinus roxburghii Sarg. might have utmost number of chemical compounds. newline The volatile oils, chloroform and methanolic extracts were screened for their antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogenes, Candida alb