EVALUATION OF ANTI CANCER ACTIVITY OF TRIGONELLA FOENUM GRAECUM METHI LEAVES FROM WESTERN HIMALAYAS AND GC MS ANALYSIS OF BIOACTIVE FRACTIONS

Abstract

newline Abstracts newlineTrigonella foenum-graecum (methi) plant is eaten in India since long. It has been mentioned in Ayurveda that Trigonella foenum-graecum was used for the treatment of wound, arthritis, bronchitis, allergies, loss of appetite, high Cholesterol, diabetes, anemia, body odour and cancer. In present study, the methanolic extracts of the methi leaves were fractionated in petroleum ether, chloroform, ethyl acetate, butanol and aqueous. Later on there fractions were screened for the presences of flavonoids, terpenoids, steroids, alkaloids, tannins, amino acids by qualitative phytochemical test. The ABTS assay was performed to analyze the antioxidant/ free radical scavenging properties of T. foenum graecum extracts. The butanol fraction showed highest free radical scavenging activity (log IC50 1.57±14.30 and#956;g/ml) as compare to other fractions and the results were comparable with that of the standard antioxidant compound ascorbic acid (log IC501.43±11.03 and#956;g/ml). Butanol fraction also showed highest amount of total phenolic content 38.48±0.05 mg gallic acid equivalent/g of dry weight of plant. T. foenum graecum leaves extract/fractions also exhibited anti-cancer activity towards breast cancer cell line (MCF-7). The chloroform fractions was found to display highest anti-cancer (log IC50 2.93±5.53 and#956;g/ml) effect towards cancer cells followed by the methanolic extracts having (log IC50 4.02±4.5 and#956;g/ml). The GC-MS analysis of the chloroform fraction revealed the presences of 20 compound and the major phytoconstituent was identified as Diisooctyl phthalate (Retention time 3.27) whereas methanolic extract contains 13 compound and major compounds were Dibutyl phathalate (Retention time 14.75) and trans 13-docosenamide (Retention time 26.54). newlineThe current work concluded that the butanol fraction of T. foenum graecum possess highest anti-oxidant due to presences of phenolic compounds. Futhermore the chloroform fractions of the T. foenum graecum displayed highest anti-cancer effect due to presences of phytocompone