In vitro Antioxidant and Antigenotoxic Potential and In silico Molecular Docking on Alpinia galanga L Willd

Abstract

Herbs are staging a comeback and a relook for herbal therapy and natural products is happening all over the globe. Alpinia galanga (L.) Willd from the family, Zingiberaceae have reported to possess significant potential to yield bioactive components towards the development of future therapeutics. In this regard, the present investigation tried to explore the antioxidant, antimutagenic and antigenotoxic newlinepotential and anticancer properties on human breast cancer cells. Preliminary phytochemical analysis revealed the presence of seven major phytochemicals viz., alkaloids, flavonoids, phenols, newlinecarbohydrates, sterols, tannins and triterpinoids. Free radial scavenging assays established that methanol extract of rhizome exhibited outstanding scavenging effects on a series of radicals. Further investigation on the enzymic and non enzymic antioxidant levels in methanol extract of rhizome newlineshowed a considerable amount of the protective enzymes especially catalase, superoxide dismutase, polyphenol oxidase and and#945;-tocopherol. The anti-mutagenic agents from the rhizome extract produced a newlineconsiderable reduction in the reverse mutation capability of the standard mutagens. In genoprotective activity, a significant reduction of chromosomal damage was observed by the addition of rhizome extract to peripheral blood lymphocyte culture, which is taken as a biomarker tool for DNA repair newlinecapacity of A.galanga. In anti-proliferative activity, a dose dependent decrease in cell viability of MCF-7 cells was observed. The UV absorption spectrum of the extract showed major and minor peaks indicating the presence of multiple components. FT-IR spectrum showed that major bioactive compounds present in the rhizome belongs to phenolic and flavonoid group of compounds. XRD analysis indicating the presence of boron enriched Aza-closo-decaborane compound. GC-MS analysis revealed the presence of 40 significant compounds including hydroxyl and carbonyl groups. From these bioactive compounds, 3-phenyl-2-butanone, nortrachelogenin and alpha terpineol