Genetic analysis of morphological biochemical and molecular traits of some novelty rices of Himachal Pradesh

Abstract

newline ABSTRACT newline newline Genetic analysis of morphological, biochemical and molecular traits of some novelty rices of Himachal Pradesh was carried out by using 17 red pericarp rices, 19 purple leaved rices and 11 quality rices along with three checks in Randomized Block Design at Rice and Wheat Research Centre, Malan (Kangra). The data was analyzed using statistical procedures. Based on overall performance, Achhoo Baldhar, IC3131159, Kijun and Tiyun among red pericarp rices; RLC-3, Lal Nakanda-41, R-575 and Purple Baldhar among purple leaved rices and LC99-5B, IC3131155 and Rajpur Basmati among quality rices were found to be promising genotypes for further exploitation. Tiyun had high sugar (10.4 mg/100g) and high protein content (14.0%). R-575 had high 1000-grain weight (32.1 g). IC3131166 and Rajpur Basmati had long slender grains, high elongation after cooking and high brown rice protein. Grain yield had significant positive correlation with grains per panicle, plant height and spikelets per panicle thus direct selection for those characters will be effective. Based on path coefficient analysis tillers per plant, panicle length, grains per panicle and 1000-grain weight had high positive direct effects on yield. Molecular analysis based on RAPD markers grouped 41 genotypes into cluster I, 25 of these were grouped into cluster II of ISSR and 33 in cluster I of D2-statistics (combined over years). This suggested similarity in clustering pattern based on these techniques. Grouping of majority of the genotypes into one cluster suggested affinity among the genotypes indicating their origin in same geographical area. Grouping based on pooling two types of markers revealed that majority of the red pericarp rices and purple leaved rices grouped into two distinct sub-clusters. Tremendous variation in anthocyanin pigmentation of different plant parts was present in all red, purple and quality rices. Among check variation was present only in case of size of auricle. Auricles were absent in Achhoo and Achhoo Baldhar. Varieties namely, China Purple, HPR-1194, HPR-2089, HPR-2178, Palampur Purple and Nagrota Purple had dark purple leaf blade, purple auricle, purple stigma and purple apiculus. Achhoo Baldhar had long grains with dark red pericarp, whereas IC3131171 and IC3131159 had light red pericarp and long grains. newline newline newline¬¬¬¬¬¬¬_______________________ _____________________ newlineSignature of student with date Signature of Major Advisor newline newlineCountersigned newline newline________________ newlineHead of Department newline newline