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dc.description.abstractAcute lung injury (ALI) or its most severe form, acute respiratory distress syndrome (ARDS) is a clinically devastating and life threatening syndrome, which is caused mainly by dysfunctioning of pulmonary surfactants. Despite advances made in understanding the intricate molecular pathogenesis of ARDS for the past 3 decades, the mortality associated with ARDS still remains at ~ 40-50%. Lipopolysaccharide (LPS), is an endotoxin, a well known inducer compound for ALI/ARDS. In order to understand its pathogenic mechanism, alteration of phospholipid metabolism in pulmonary system in the current work is well studied. Previous reports have focussed mainly on phosphatidylcholine (PC), especially dipalmitoyl-PC (DPPC) and phosphatidylglycerol (PG) but the regulation of phospholipids in lungs during endotoxemia have not been elucidated. The immunological effect of LPS is also well documented in spleen and thymus; however impact on lipids particularly phospholipid metabolism is yet to be studied. Hence the present study was undertaken to investigate the role of phospholipids in lung, spleen and thymus during ARDS. Our objective was first initiated by in vitro metabolic labelling studies with [32P]orthophosphate followed by in vivo experiments. Labelling studies revealed significant impairments in PC and PG of lung, spleen and PC in thymus. The fatty acid (FA) analysis also showed marked alterations. To further understand the PL metabolism we have developed an animal model for ALI/ARDS [Sabarirajan et al., 2010]. Rats were divided into four groups. Group I, Control animals (24 h saline treated); Group II, III and IV were given LPS (10 mg/kg body wt.) intraperitoneally and sacrificed after 6, 12 and 24 h respectively. We observed reduced phospholipid content especially PC, PG in lung and major PL in spleen and thymus. FA distribution of individual PL shows an overall increase in unsaturated/saturated FA in lung, spleen and thymus. This might be due to the sensitive/adaptive response of the organ.en_US
dc.titleImpaired phosphoglyceride metabolism in lung, spleen and thymus during lipopolysaccaride - induced acute respiratory distress syndrome in male wistar ratsen_US
dc.creator.researcherSabariragian, Jayarajen_US
dc.subject.keywordFatty acidsen_US
dc.subject.keywordRemodeling enzymeen_US
dc.description.noteBibliography p.123-141en_US
dc.contributor.guideVasanthi Nachiappanen_US
dc.publisher.universityBharathidasan Universityen_US
dc.publisher.institutionDepartment of Biochemistryen_US
Appears in Departments:Department of Biochemistry

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05_abbreviations.pdf41.19 kBAdobe PDFView/Open
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07_abstract.pdf83.66 kBAdobe PDFView/Open
08_chapter 1.pdf926.83 kBAdobe PDFView/Open
09_chapter 2.pdf2.03 MBAdobe PDFView/Open
10_chapter 3.pdf1.67 MBAdobe PDFView/Open
11_bibliography.pdf250.29 kBAdobe PDFView/Open

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