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Title: Molecular characterization of begomovirus causing leaf curl disease in sunflower
Researcher: Vanitha L S
Guide(s): Rangaswamy K T
Keywords: Agricultural Sciences
Leaf curl disease
Upload Date: 8-Jan-2013
University: University of Agricultural Sciences, Bangalore
Completed Date: 28/08/2012
Abstract: Sunflower (Helianthus annuus L.) is one of the three important edible oilseed crops grown in the world after soybean and groundnut. The crop has been found suffering from many diseases. Recently a leaf curl disease caused by a begomovirus has been observed in Northern Karnataka, India. Sunflower leaf curl virus infected plant are associated with symptoms such as vein clearing, vein thickening, upward curling of newlineleaves, reduction in the leaf size, enations, stunted growth with reduced ear head resulted poor seed set. The occurance of disease incidence up to (58.20%) in MARS, Pamanakallur (10%) of Raichur district. Murdi (10%), Narasapur (25%), Kanakagiri (12.50%), Somanakoppa (16%), Mangaluru (25%), Beur (15.50%), Gundlanur (8%), Betagere (10.50%), Katarki (20%) disease incidence was noticed in villages of Koppal District, Northren newlineKarnataka. Twenty viruliferous whiteflies were required for 100 per cent transmission of SuLCV. Among twelve plant species tested, SuLCV was successfully infected the plant species viz, tobacco, tomato, Zinnia, newlineParthenium and Acanthospermum sp. The virus was detected using CP specific primers which amplified ~570 bp product of core CP of the viral genome which was cloned and sequenced. The sequences shared highest coat protein nucleotide identity (97.5%) with ToLCKV-(Luc) and ToLCBV- (Ban2) isolates. The complete nucleotide sequence of DNA A component was determined to be 2761 nucleotides. Full genome (DNA-A) Sequence of SuLCV showed maximum identity with ToLCKV and ToLCV-[Ban-II] isolates. SuLCV beta DNA satellite molecule of ~1.3 kb was amplified and newlinesequence consisted of 1373 nt. Maximum nucleotide sequence identity of (94.07%) was with Potato apical leaf curl disease associated satellite DNA beta. The alpha DNA satellite molecule was amplified and the sequence consisted of 1350 nt showed maximum nucleotide sequence identity of (94%) with Tobacco curly shoot alpha DNA satellite molecule associated with leaf curl disease on wild sunflower.
Pagination: 93p.
Appears in Departments:Department of Plant Pathology

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01_title.pdfAttached File87.48 kBAdobe PDFView/Open
02_certificate.pdf159.81 kBAdobe PDFView/Open
03_acknowledgement & abstract.pdf776.4 kBAdobe PDFView/Open
04_contents.pdf95.47 kBAdobe PDFView/Open
05_list of tables figures & plates.pdf145.06 kBAdobe PDFView/Open
06_chapter 1.pdf36.86 kBAdobe PDFView/Open
07_chapter 2.pdf120.21 kBAdobe PDFView/Open
08_chapter 3.pdf469.6 kBAdobe PDFView/Open
09_chapter 4.pdf1.59 MBAdobe PDFView/Open
10_chapter 5.pdf65.79 kBAdobe PDFView/Open
11_chapter 6.pdf52.75 kBAdobe PDFView/Open
12_references.pdf3.2 MBAdobe PDFView/Open

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