Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/3456
Title: New analytical methods development and identification and characterization of impurities in pharmaceuticals
Researcher: Ankam, Rajesh
Guide(s): Mukkanti, K
Durga Prasad, A
Keywords: Chemistry
Orlistat
Pharmaceuticals
Upload Date: 19-Apr-2012
University: Jawaharlal Nehru Technological University
Completed Date: May, 2010
Abstract: An accurate and precise gradient stability indicating reverse phase high performance liquid chromatographic method is developed for simultaneous determination of sibutramine hydrochloride and Orlistat in the newly developed capsule formulation. The chromatography equipped with Zorbax SB Phenyl (250×4.6mm) 5µ column using a mobile phase of buffer (Dissolved 7.0 g of sodium perchlorate in 1000ml water, pH adjusted to 2.1 (+ 0.05) with ortho phosphoric acid, filtered through 0.45 µ filter.) and acetonitrile, the gradient program configured with a flow rate of 2.0 ml/min, ultraviolet detector at 210nm. The retention times of sibutramine hydrochloride and Orlistat were 8 and 31 min respectively. The method linearity was studied over the range of 50 to 150 ppm with r2 of 0.9999 for sibutramine hydrochloride and 600 to 1800 ppm with r2 of 0.9999 for Orlistat. Mean recovery for sibutramine hydrochloride and Orlistat were 100.09 and 100.02 respectively A simple fast, accurate, precise, and cost effective isocratic stability indicating reverse phase high performance liquid chromatographic method is developed for simultaneous determination of Halobetasol propionate and fusidic acid as well as its impurities in the newly developed cream formulation. The chromatography equipped with Zorbax SB phenyl (250X4.6mm) column using a mobile phase of buffer (0.1% triethylamine buffer pH adjusted to 2.0 with Orthophosphoric acid) acetonitrile, methanol in the ratio of 40:35:25 v/v with a flow rate of 1.5 ml/min, with ultraviolet detector configured at 240nm. Identified all impurities and Characterization of Sibutramine Hydrochloride Impurities.
Pagination: 268p.
URI: http://hdl.handle.net/10603/3456
Appears in Departments:Faculty of Chemistry

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01_title.pdfAttached File308.56 kBAdobe PDFView/Open
02_certificates.pdf261.89 kBAdobe PDFView/Open
03_acknowledgements.pdf245.3 kBAdobe PDFView/Open
04_abstract.pdf172.95 kBAdobe PDFView/Open
05_table of contents.pdf272.32 kBAdobe PDFView/Open
06_list of tables & figures.pdf290.66 kBAdobe PDFView/Open
07_abbreviations.pdf225.25 kBAdobe PDFView/Open
08_chapter 1.pdf334.5 kBAdobe PDFView/Open
09_chapter 2.pdf3.34 MBAdobe PDFView/Open
10_chapter 3.pdf532.95 kBAdobe PDFView/Open
11_chapter 4.pdf673.29 kBAdobe PDFView/Open
12_chapter 5.pdf489.72 kBAdobe PDFView/Open
13_chapter 6.pdf682.86 kBAdobe PDFView/Open
14_chapter 7.pdf427.89 kBAdobe PDFView/Open
15_references.pdf494.97 kBAdobe PDFView/Open
16_research publications.pdf236.75 kBAdobe PDFView/Open


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