Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/344709
Title: Biotechnological Investigation of lignolytic Potential of Rigidoporus sp DK4
Researcher: Kale Swapnil Kisanrao
Guide(s): Patil Vikram Bapurao
Keywords: Biology
Biology and Biochemistry
Life Sciences
University: Swami Ramanand Teerth Marathwada University
Completed Date: 2020
Abstract: Laccase has wide application in industries like textile dye bleaching, pulping and bioremediation, due to broad substrate specificity and redox potential. With advancement in biotechnology industry, fungi have been in use for commercial laccase production. Strain improvement is an essential part of process development for microbial products, to be economically feasible. It can reduce costs by developing strains with increased productivity. The strain improvement intended to increase production of desirable enzymes by mutant strains. Fungal strain improvement to increase production of enzymes has been the feature of all viable fermentation processes, such superior strains can be cost effective with better productivity. Present study, proposed the possibility for the exploitation of the Rigidoporus sp. DK4 and its mutants for production of laccase under SmF and SSF. So, present study was carried out to enhance the production of laccase by means of chemical and physical mutagens. newlineThe effects of various mutagens such as acrylamide, colchicines, ethylmethane sulphonate, ethidium bromide and gamma radiations on laccase production by Rigidoporus sp. DK4 were investigated. All chemical and physical mutagens have more distinct effect on its growth rate, higher concentration of mutagen may have lethal effect on treated fungi. All the mutagens showed varying influence on fungal growth. Screening of novel mutants was carried out using plate assay, based on guaiacol oxidation and polymeric dye decolourization. All mutants showed promising resluts in screening. In terms of laccase activity, no linear relationship observed with respect to concentrations of mutagens. Highest laccase producing mutant of Rigidoporus sp. DK4 (EMS treatment at 200and#956;g/ml) observed to produce 4.84 fold increase in laccase activity (34560U/L), therefore EMS-200 mutant strain was selected for further optimization study. newlineRigidoporus sp. DK4 cultivated under submerged fermentation condition to optimize culture conditions for induction of hyper laccase prod
Pagination: 140p
URI: http://hdl.handle.net/10603/344709
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File217.73 kBAdobe PDFView/Open
02_certificate.pdf163.2 kBAdobe PDFView/Open
03_abstract.pdf214.5 kBAdobe PDFView/Open
04_declaration.pdf244.5 kBAdobe PDFView/Open
05_acknowledgment.pdf248.68 kBAdobe PDFView/Open
06_contents.pdf257.89 kBAdobe PDFView/Open
07_list_of_tables.pdf273.53 kBAdobe PDFView/Open
08_list_of_figures.pdf417 kBAdobe PDFView/Open
09_abbreviations.pdf393.68 kBAdobe PDFView/Open
10_chapter 01.pdf405.24 kBAdobe PDFView/Open
11_chapter 02.pdf881.06 kBAdobe PDFView/Open
12_chapter 03.pdf511.75 kBAdobe PDFView/Open
13_chapter 04.pdf5.15 MBAdobe PDFView/Open
14_chapter 05.pdf501.89 kBAdobe PDFView/Open
15_summary.pdf350.32 kBAdobe PDFView/Open
16_bibliography.pdf570.98 kBAdobe PDFView/Open
80_recommendation.pdf559.29 kBAdobe PDFView/Open


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