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Title: Formulation Development and Characterization of Targeted Nano Drug Delivery Systems for the Teatment of Tuberculosis
Researcher: Chokshi, Nimitt
Guide(s): Patel, Mayur
Keywords: Clinical Pre Clinical and Health
zeta potential
University: Nirma University
Completed Date: 2020
Abstract: The oral delivery of anti-tubercular drugs remains challenging, despite being the most compliant route of administration. The main aim of current investigation demonstrates development of lung targeted surface tailored drug loaded solid lipid nanoparticles comprising of first line anti-TB agents. These drug loaded SLNs were developed and optimized by applying Design of Experiments. Significant independent variables like concentration of drug, concentration of emulsifier, homogenization cycles and homogenization pressure were identified and evaluation was statistically performed on dependent variables like %drug loading (%DL), newline%entrapment efficiency (%EE) and particle size. These developed drug loaded SLNs were characterized for particle size, polydispersity index (PDI), zeta potential (ZP), %EE, %DL, DSC, FTIR and TEM analysis. These were further surface modified by coupling of mannose to the amine group of stearylamine-functionalized SLNs. The in vitro dissolution studies were performed and kinetic profile for drug loaded SLNs revealed mechanistic explanation mainly through diffusion and dissolution mechanism. The in vitro cytotoxicity studies were performed using J774A.1 cell line depicting that the developed SLNs were non-toxic and safe as compared to pure drug solution individually. Fluorescence imaging and flow cytometric analysis revealed remarkable intracellular uptake of coumarin-6 (fluorophore) loaded mannosylated SLNs at 9 h for all formulations. The in vivo pharmacokinetic studies in rats revealed that the mannosylated SLNs showed significant increase in relative bioavailability as compared to pure drug solution following oral administration. The in vivo biodistribution profiles revealed higher lung accumulation by Mannosylated SLNs as compared to unconjugated SLNs. Thus, it can be concluded that the developed drug loaded mannosylated SLNs could be used as a potential formulation for the treatment of tuberculosis. newline newlineKeywords: solid lipid nanoparticles; targeted drug delivery; mannose
Appears in Departments:Institute of Pharmacy

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13_chapter_4.pdf6.26 MBAdobe PDFView/Open
14_chapter_5.pdf5.24 MBAdobe PDFView/Open
15_chapter_6.pdf6.08 MBAdobe PDFView/Open
16_chapter_7.pdf197.15 kBAdobe PDFView/Open
17_list of poster and publications.pdf179.37 kBAdobe PDFView/Open
1_title.pdf120.4 kBAdobe PDFView/Open
2_certificate.pdf66.25 kBAdobe PDFView/Open
3_declaration.pdf70.42 kBAdobe PDFView/Open
4_table of contents.pdf144.45 kBAdobe PDFView/Open
5_acknowledgements.pdf148.81 kBAdobe PDFView/Open
6_abstract.pdf144.68 kBAdobe PDFView/Open
80_recommendation.pdf473.73 kBAdobe PDFView/Open
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