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Title: Investigation on anti pathogenic potential of Herboheal formulation and Phyllanthus emblica L seed extract against selected human pathogenic bacteria
Researcher: Patel, Pooja
Guide(s): Kothari, Vijay
Keywords: bacterium
Biology and Biochemistry
Life Sciences
University: Nirma University
Completed Date: 2020
Abstract: In context of the global threat of antimicrobial resistance (AMR) among pathogenic bacteria against conventional bactericidal antibiotics, investigation on complementary/ alternative approaches to manage bacterial infections is urgently warranted. Present study aimed at investigating the anti-pathogenic potential of Herboheal formulation (HF; a polyherbal formulation), and Phyllanthus emblica seed extract (PESE) against four different pathogenic bacteria viz. three antibiotic-resistant gram-negative bacteria- Chromobacterium violaceum, Serratia marcescens, Pseudomonas aeruginosa (latter two are #CDC-listed), and one gram-positive bacterium Staphylococcus aureus. This study has found both the extracts to be effective broad-spectrum quorum modulators, as they could affect production of QS (quorum sensing)-regulated pigments and other virulence traits in gram-positive as well as gram-negative bacterial pathogens. PESE and HF were found to exert in vitro quorum-modulatory effect on these pathogens at and#8805;50 and#956;g/mL and and#8805;0.025%v/v respectively. These extracts could curb the hemolytic activity of all the four test bacteria by 18-69%. Test formulations not only could inhibit (24-55%) biofilm formation, but also eradicated (18-63%) pre-formed biofilm effectively, and were also able to modulate bacterial antibiotic susceptibility and catalase activity (5-21%). Susceptibility of P. aeruginosa and S. aureus to lysis by human serum was enhanced under the influence of these extracts by 23-86%. Repeated exposure of both these notorious pathogens to PESE or HF did not induce resistance in them. In vivo assay demonstrated the ability of these extracts of attenuating (7-70%) bacterial virulence towards the model host C. elegans. Whole transcriptome analysis revealed certain genes associated with hemolysis, virulence, enzyme activity, transport, basic cellular processes (e.g. RNA metabolism), quorum sensing, and transcriptional regulators as the major targets of Herboheal in S. aureus. Both the extracts also showed notable p
Appears in Departments:Institute of Science

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02_certificate.pdf62.83 kBAdobe PDFView/Open
03_ abstract.pdf257.71 kBAdobe PDFView/Open
04_declaration.pdf67.75 kBAdobe PDFView/Open
05_acknowledgement.pdf103.17 kBAdobe PDFView/Open
06_ contents.pdf206.56 kBAdobe PDFView/Open
07_list of tables.pdf259.26 kBAdobe PDFView/Open
08_ list of figures.pdf155.6 kBAdobe PDFView/Open
09_list of abbreviations.pdf364.86 kBAdobe PDFView/Open
10_chapter_1.pdf582.79 kBAdobe PDFView/Open
11_chapter_2.pdf1.19 MBAdobe PDFView/Open
12_chapter_3.pdf931.83 kBAdobe PDFView/Open
13_chapter_4.pdf4.36 MBAdobe PDFView/Open
14_conclusion.pdf274.58 kBAdobe PDFView/Open
15_ summary.pdf460.15 kBAdobe PDFView/Open
16_ references.pdf518.93 kBAdobe PDFView/Open
80_recommendation.pdf173.92 kBAdobe PDFView/Open
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