Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/323868
Title: Role of Gold Nanoparticles Towards Enhancement of Antimelanoma Bioefficacy of the Plant Madhuca Longifolia
Researcher: Srivastava, M. M. and Ganesh, N.
Guide(s): Yadav, Saurabh
Keywords: Chemistry
Chemistry Organic
Physical Sciences
University: Dayalbagh Educational Institute
Completed Date: 2020
Abstract: The present thesis is devoted towards the phyto-fabrication and characterization of gold nanoparticles loaded with flavonoids, extracted from the plant Madhuca longifolia with special reference to its in-vitro and in-vivo anti-melanoma bio-efficacy. The work highlights that among the various parts of the plant Madhuca longifolia, its bark portion contains a significant amount of total phenolics and flavonoids content. The synergistic redox potential of the flavonoid content, extracted from the bark of the plant is capable of phyto-fabrication of gold nanoparticles loaded with the flavonoid in a simple green singular process. Phyto-fabricated gold nanoparticles (FatAuNp) are of face centred cubic structure, spherical/ cubic shape, size range (9.66 -17.58 nm) with an average roughness of 14.15 nm, Zeta size 17.58 nm with poly-dispersive index 0.358 and Zeta potential -25.7 mV. Radioisotopic studies established the stay period of FatAuNp in a living system for six hours in contrast with that of native flavonoid biomolecules having a short stay period. The capping effect of flavonoid onto the freshly generated gold nanoparticles has been proved using HPTLC fingerprinting. In-vitro anti-melanoma bio-efficacy of extracted flavonoid content (77.14 at 15µg/ml) in mice melanoma (B16F10) cell lines have been successfully enhanced to (82.75% at 15 µg/ml) using FatAuNp. It is noteworthy that FatAuNp exhibited significant anti-melanoma bio-efficacy without showing any cytotoxicity towards human lymphocytes cells and myelosuppression activity as noticed in reference drug (cyclophosphamide). The explored promising anti-melanoma activity of the plant Madhuca longifolia opens a window for the development of effective herbal nano-medicine for the treatment of melanoma cancer, triggering cellular apoptosis, necrosis and decreased angiogenesis. newline
Pagination: 
URI: http://hdl.handle.net/10603/323868
Appears in Departments:Department of Chemistry

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01_title.pdfAttached File81.61 kBAdobe PDFView/Open
02_certificates.pdf130.96 kBAdobe PDFView/Open
03_declaration.pdf174.43 kBAdobe PDFView/Open
04_abstract.pdf84.78 kBAdobe PDFView/Open
05_acknowledgement.pdf136.52 kBAdobe PDFView/Open
06_contents.pdf117.46 kBAdobe PDFView/Open
07_list_of_tables.pdf87.79 kBAdobe PDFView/Open
08_list_of_figures.pdf114.69 kBAdobe PDFView/Open
09_abbrebrations.pdf136.04 kBAdobe PDFView/Open
10_chapter 1.pdf434.37 kBAdobe PDFView/Open
11_chapter 2.pdf573.17 kBAdobe PDFView/Open
12_chapter 3.pdf277.37 kBAdobe PDFView/Open
13_chapter 4.pdf173.85 kBAdobe PDFView/Open
14_chapter 5.pdf947.74 kBAdobe PDFView/Open
15_chapter 6.pdf2.8 MBAdobe PDFView/Open
16_conclusion.pdf141.28 kBAdobe PDFView/Open
17_references.pdf302.6 kBAdobe PDFView/Open
18_appendix.pdf89.78 kBAdobe PDFView/Open
19_summary.pdf88.72 kBAdobe PDFView/Open
80_recommendation.pdf303.04 kBAdobe PDFView/Open


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