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Title: A comparative study on virulence factors of Candida albicans under hyphal form induced by different inducers
Researcher: Radfan Ahmed Abdulghani
Guide(s): Zore G B
Keywords: Biochemistry and Molecular Biology
Biology and Biochemistry
Life Sciences
University: Swami Ramanand Teerth Marathwada University
Completed Date: 2019
Abstract: Candida albicans exhibit eight genes in ALS gene family (Agglutinin-like sequence) that encode eight large cell-surface glycoproteins involved in adhesion to host cells. We have made an attempt to understand expression pattern of eight ALS family genes under hyphal form induced by environmental (Temperature (37and#730;C) and neutral pH) and nutritional (Serum, Proline, glucose and N-acetyl glucosamine) factors. Expression analysis was also correlated with structural peculiarities of ALS family genes and proteins using in silico analysis. Based on expression analysis, we could group these ALS family genes in three groups i.e. gene expressed under yeast form, hyphal form and yeast and hyphal form specific while third group is microenvironment and morphological form specific. Wherein genes were over-expressed in yeast (ALS1, ALS2 and ALS4) and hyphal (ALS3) form respectively. Third group is modulated by both microenvironment and morphological form (ALS5, ALS7 and ALS9). In general, ALS3 gene is the only gene which over-expresses under hyphal form and work as an adhesin (invasin). As the microenvironmental conditions used in this study mimic some of the In vivo microenvironment, our data can be correlated with in vivo expression of ALS family genes. Cell surface hydrophobicity increased in response to inducers at 30and#778; C while decreased at 37and#778; C in response to all inducers indicating that there is a correlation between cell surface hydrophobicity and adherence. We have made an attempt to understand structural/ molecular peculiarities of ALS family genes, using in silico analysis to correlate their over-expression under those microenvironmental conditions. However further studies are needed to validate the claim. newline
Pagination: 220p
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File245.24 kBAdobe PDFView/Open
02_certificate.pdf202.23 kBAdobe PDFView/Open
03_abstract.pdf182.69 kBAdobe PDFView/Open
04_declaration.pdf192.97 kBAdobe PDFView/Open
06_contents.pdf361.9 kBAdobe PDFView/Open
07_list_of_tables.pdf180.77 kBAdobe PDFView/Open
08_list of_figures.pdf183.25 kBAdobe PDFView/Open
09_abbreviations.pdf88.67 kBAdobe PDFView/Open
10_chapter 1.pdf195.12 kBAdobe PDFView/Open
11_chapter 2.pdf528.79 kBAdobe PDFView/Open
12_chapter 3.pdf176.51 kBAdobe PDFView/Open
13_chapter 4.pdf230.79 kBAdobe PDFView/Open
14_chapter 5.pdf1.72 MBAdobe PDFView/Open
15_chapter 6.pdf360.49 kBAdobe PDFView/Open
16_summary.pdf202.78 kBAdobe PDFView/Open
17_conclusion.pdf179.21 kBAdobe PDFView/Open
18_bibliography.pdf346.01 kBAdobe PDFView/Open
80_recommendation.pdf620.38 kBAdobe PDFView/Open

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