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Title: Studies on pseudomonas aeruginosa lipase secretion and molecular dynamics simulation
Researcher: Subbalakshmi latha C
Guide(s): Gautam P
Keywords: aeruginosa lipase secretion
molecular dynamics simulation
science and humanities
Upload Date: 7-Oct-2014
University: Anna University
Completed Date: 01/04/2006
Abstract: Lipases triacylglycerol hydrolases EC 3113 catalyze the newlinehydrolysis of triglycerides to free fatty acids and glycerol Extracellular lipase newlinesecreted by the gramnegative bacterium Pseudomonas aeruginosa is a newlinepromising candidate for biotechnological applications and is extensively used newlinefor chiral production of racemic compounds newlineIn the first part of this thesis the production of lipase by newlinePseudomonas aeruginosa PGSL 03 isolated from surfactant gel utilizing newlinevegetable oil seeds was investigated Maximum lipase activity was observed newlineat 30C and pH 75 in sesame meal at 2 wv concentration The amount of newlinelipase correlated well with the relative percentage of calcium and C18 n fatty newlineacid esters present in the media The study therefore identifies vegetable oil newlineseeds as a simple and economical lipidrich media viable at an industrial newlinescale Ammonium Sulphate precipitation of culture supernatant resulted in newlinepartial purification of the enzyme 428 fold with 906 yield newline newline
Pagination: xxiii,126p.
Appears in Departments:Faculty of Science and Humanities

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01_title.pdfAttached File14.24 kBAdobe PDFView/Open
02_certificate.pdf7.61 kBAdobe PDFView/Open
03_abstract.pdf52.44 kBAdobe PDFView/Open
04_acknowledgement.pdf7.69 kBAdobe PDFView/Open
05_contents.pdf121.97 kBAdobe PDFView/Open
06_chapter 1.pdf688.24 kBAdobe PDFView/Open
07_chapter 2.pdf679.1 kBAdobe PDFView/Open
08_chapter 3.pdf679.11 kBAdobe PDFView/Open
09_chapter 4.pdf127.75 kBAdobe PDFView/Open
10_references.pdf122.43 kBAdobe PDFView/Open
11_publications.pdf8.94 kBAdobe PDFView/Open
12_vitae.pdf5.46 kBAdobe PDFView/Open

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