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Title: DNA Barcoding And Phylogeny Based Comparative Evaluation Of Anti Cancer Properties Of Caulerpa J V Lamouroux spp From Indian Coasts
Researcher: Mehra, Richa
Guide(s): Bast, Felix
Keywords: Caulerpa, barcoding, cancer, phylogeny, anticancer
Life Sciences,Biology and Biochemistry,Biochemistry and Molecular Biology
University: Central University of Punjab
Completed Date: 23/07/2019
Abstract: iv newlineABSTRACT newlineDNA barcoding and phylogeny based comparative evaluation of anti-cancer newlineproperties of Caulerpa (J V Lamouroux) spp. from Indian coasts newlineName of Student Richa Mehra newlineRegistration Number CUPB/MPHIL-PHD/SBAS/BIO/2012-13/05 newlineDegree for which submitted Doctorate of Philosophy newlineName of Supervisor Dr. Felix Bast newlineName of Co-Supervisor Dr. Sandeep Singh newlineDepartment Centre for Biosciences newlineSchool of Studies School of Basic and Applied Sciences newlineKeywords Caulerpa, barcoding, cancer, phylogeny, anticancer newlineA total of 15 Caulerpa samples were collected from Indian coasts and newlineidentified based on morphological and molecular data inferred from ITS, 18S, tufA newlineand rbcL. Seven different species viz. C. scalpelliformis, C. racemosa, C. newlinesertularioides, C. verticillata, C. taxifolia, and C. corynephora; and their geographical newlineisolates were identified. Barcode data for these species was generated using newlineaforementioned molecular markers and used for phylogenetic assessment. newlinePhylogenetic trees using Bayesian inference (BI) and Maximum Likelihood (ML) newlinefunction were generated for each molecular marker. tufA was found to be most newlinesuitable marker for the genus Caulerpa, resolving the species into 17 different newlinelineages, with 15 corresponding to already known sections and 2 new lineages. newlineBesides, a database named DbIndAlgae of Indian algae was generated and all the newlinemorphological as well as molecular data generated in this study is uploaded on the newlinedatabase. In addition, the phycochemical analysis revealed the presence of newlinealkaloids, terpenoids, steroids, tannins, saponins, flavonoids, and phenols in newlinedifferent Caulerpa species. The selective cytotoxicity of methanolic extracts of newlineCaulerpa (CMEs) was evaluated on MDA-MB-231, T47-D and H1299 cells, and the newlineresults revealed significant cytotoxicity of all species. C. racemosa KNY-254 and C. newlinetaxifolia TEN-158 were found to be most potent on MDA-MB-231 cells with IC50 newlinevalue of 0.226 ± 0.004 and 0.246 ± 0.009 and#956;g/and#956;L. The mitochondrial membrane newlineperturbation was revealed by JC-1 and apoptotic cell death
Appears in Departments:Department of Bioscience

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01_title.pdfAttached File44.58 kBAdobe PDFView/Open
02_certificate 1.pdf25.12 kBAdobe PDFView/Open
03_certificate 2.pdf25.13 kBAdobe PDFView/Open
04_abstract.pdf35.48 kBAdobe PDFView/Open
05_acknowledgements.pdf46.72 kBAdobe PDFView/Open
06_table of contants.pdf64.13 kBAdobe PDFView/Open
07_introduction.pdf41.94 kBAdobe PDFView/Open
08_review of literature.pdf544.98 kBAdobe PDFView/Open
09_materials and methods.pdf437.09 kBAdobe PDFView/Open
10_results.pdf9.24 MBAdobe PDFView/Open
11_discussion.pdf97.71 kBAdobe PDFView/Open
12_references.pdf192.66 kBAdobe PDFView/Open
13_appendix.pdf234.5 kBAdobe PDFView/Open
14_list of publications.pdf47.16 kBAdobe PDFView/Open

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