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Title: DNA barcode based identification and comparative anti cancer effects of different species of brown seaweed Sargassum C Agardh of Indian coasts
Researcher: Bhushan, Satej
Guide(s): Bast, Felix and Singh, Sandeep
Keywords: Sargassum, macro-algae, phylogeny, cancer, anti-cancer activity
University: Central University of Punjab
Completed Date: 17/09/2018
Abstract: Sargassum C. Agardh is a ubiquitous, multicellular brown seaweed that represents newlinethe most species-rich genus of the brown algal order Fucales, with more than 500 newlinespecies reported worldwide. The present study aimed to identify different newlineSargassum isolates from India by DNA barcoding of mitochondrial (cox3), newlinechloroplast (rbcL), and nuclear (18S) regions and further phylogenetic analyses. newlineTotal of 17 geographical isolates were collected across Indian coasts. Phylogeny newlinereconstruction using Bayesian Inference was done which suggested congruency newlinewith known taxonomic hierarchy of Sargassum. Total of five different species were newlineidentified (S. portierianum, S. cymosum, S.aquifolium, S. ilicifolium, S. polycystum). newlineIn addition, comparative evaluation of anti-cancer potential of all the isolates was newlinecarried out and putative relationship between phylogeny and anticancer potential newlinewas established. MTT assay with 3 different cell lines showed cytotoxicity with IC50 newlineas low as 0.167 ± 0.01, 0.243 ± 0.007, 0.25 ± 0.03 and#956;g/and#956;L in MDA-MB-231 (Breast newlineCancer), T-47D (Breast Cancer), H1299 (Lung Cancer) cells respectively, while no newlinetoxicity was observed with human peripheral blood mononuclear cells (hPBMCs). I newlinewas also able to isolate one lead aliphatic compound (SA1) whch was identified to newlinebe a polysaccharide using NMR spectroscopy. Similar to the extract, purified newlinecompound SA1 also showed anticancer activity. Further evaluations revealed that newlineSA1 as well as the extracts interfere with the antioxidant defence components of newlinecancer cells (SOD, Catalase, and GR) which results in the induction of mitochondrial newlinedeath pathway at G1 phase (for extracts) as well as at G2M phase (for SA1). newlineExtracts as well as SA1 were also able to inhibit cancer cell migration at sub IC50 newlinedoses. In addition, sub IC50 treatments lead to decreased colony formation newlinecompared to the control. Overall, our results show that these extracts as well as SA1 newlineare able to target multiple properties of cancer newline
Appears in Departments:Department of Bioscience

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01_title.pdfAttached File75.83 kBAdobe PDFView/Open
02_certificate.pdf106.05 kBAdobe PDFView/Open
03_abstracts.pdf46.48 kBAdobe PDFView/Open
04_acknowledgement.pdf85.36 kBAdobe PDFView/Open
05_table of contents.pdf42.71 kBAdobe PDFView/Open
06_list of tables.pdf45.08 kBAdobe PDFView/Open
07_list of figures.pdf116.81 kBAdobe PDFView/Open
08_list of abbreviations.pdf107.81 kBAdobe PDFView/Open
09_list of appendices.pdf41.14 kBAdobe PDFView/Open
10_chapter 1.pdf110.25 kBAdobe PDFView/Open
11_chapter 2.pdf556.9 kBAdobe PDFView/Open
12_chapter 3.pdf446.74 kBAdobe PDFView/Open
13_chapter 4.pdf4.87 MBAdobe PDFView/Open
14_chapter 5.pdf143.76 kBAdobe PDFView/Open
15_summary.pdf46.87 kBAdobe PDFView/Open
16_references.pdf206.51 kBAdobe PDFView/Open
17_appendices.pdf356 kBAdobe PDFView/Open

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