Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/209077
Title: UV B PROTECTANT ACTIVITY AND THE EFFECT OF PLANT EXTRACTS ON THE REGULATION OF HAEM OXYGENASE 1 GENE VIA Nrf2 AND ANTIOXIDANT RESPONSE ELEMENT
Researcher: PATWARDHAN SANJAY JUILEE
Guide(s): BHATT PURVI
Keywords: Biological consequences of UV radiation
Damage to Biomolecules
DNA
Nitrogen Species
Oxygen Species
Skin
Solar UV Radiation
University: Narsee Monjee Institute of Management Studies
Completed Date: 2016
Abstract: The exposure of skin to ultraviolet-B (UV-B) radiations leads to DNA damage and excessive production of free radicals which imbalance the redox status of the cell and lead to increased oxidative stress. One of the important pathways in antioxidant defence is the Nrf2-ARE pathway which scavenges the oxidants and maintains the redox homeostasis of the cell. Eugenia caryophylata (clove), Foeniculum vulgare (fennel seed) and Abelmoschus esculentus (okra) has been reported to have high polyphenolic content, antioxidant potential and their medicinal properties are well known in traditional medicine. In the current study, UV-B protective abilities of flavonoids from clove, fennel seed and okra and their role in reducing oxidative stress through Nrf2-ARE pathway was determined in human dermal fibroblast (HDF) cells. Protective ability of flavonoid enriched fractions of clove, fennel seed and okra were studied against UV-B induced cytotoxicity, antioxidant regulation, oxidative DNA damage, intracellular reactive oxygen species (ROS) generation, apoptotic morphological changes and regulation of HO-1 gene through Nrf2-ARE pathway. Results demonstrated that flavonoid enriched fractions exhibited a significant antioxidant potential, flavonoid content and protected against UV-B induced cytotoxicity, depletion of endogenous enzymatic antioxidants, oxidative DNA damage, intracellular ROS production, apoptotic changes and over-expression of Nrf2 and HO-1. The present study demonstrated for the first time that the flavonoid enriched fractions of clove, fennel seed and okra could confer UV-B protection probably through Nrf2-ARE pathway by down-regulating Nrf2 and HO-1. These findings suggested that the flavonoids from clove, fennel seed and okra could potentially be considered as UV-B protectants and can be explored further for its topical application to the area of the skin requiring protection. newline
Pagination: x, 198
URI: http://hdl.handle.net/10603/209077
Appears in Departments:Department of Biological Sciences

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01_ title pages and acknowledgements.pdfAttached File316.46 kBAdobe PDFView/Open
02_ index.pdf128.49 kBAdobe PDFView/Open
03_ abbre, list of tables and figures.pdf101.54 kBAdobe PDFView/Open
04_ abstract.pdf7.09 kBAdobe PDFView/Open
05_ chapter 1.pdf965.22 kBAdobe PDFView/Open
06_ chapter 2.pdf149.64 kBAdobe PDFView/Open
07_ chapter 3.pdf502.79 kBAdobe PDFView/Open
08_ chapter 4.pdf410.9 kBAdobe PDFView/Open
09_ chapter 5.pdf1.83 MBAdobe PDFView/Open
10_ chapter 6.pdf811.86 kBAdobe PDFView/Open
11_ chapter 7.pdf284.85 kBAdobe PDFView/Open
12_ references.pdf321.89 kBAdobe PDFView/Open


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