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Title: Role of Stress and Stress Hormones on ovulation and implantation in mice Involvement of Adrenals
Researcher: Kala M
Guide(s): Nivsarkar Manish
Keywords: Adrenal
University: Nirma University
Completed Date: 02/06/2017
Abstract: Studies have revealed that stressful events in women s life may suppress the reproductive functions viz., pre-fertilization (copulation, oocyte maturation, ovulation), gestational (implantation, placentation, organogenesis) and post partum (pregnancy rate, live birth delivery, birth weight etc.). However, the exact mechanism underlying is largely unknown. In the female reproductive system, superoxide anion radical and superoxide dismutase (SOD) enzymes play vital role in the ovulation process, oocyte development, progesterone production and implantation. newlineThe present study was designed to explore the plausible mechanism of action of psychological stress on ovulation and implantation by targeting SOD activity. Initially a psychological stress model was developed and optimized in mice by full factorial design which showed increased cortisol level along with behavioural changes viz., anhedonia, depression and anxiety. Exposure to psychological stress model resulted in irregular estrous cyclicity with increased number of atretic antral follicle and pyknotic granulosa cell in mice. Also, the total SOD activity and expression of SOD1 and SOD2 was upregulated in stressed mice during estrous cycle stages. Hormone assay showed increased Luteinizing hormone and progesterone level in stressed cycling mice. The mice were also superovulated to understand the effect of stress on oocyte maturation and it was observed that only immature oocytes were obtained in stressed mice. newlineImplantation is yet another important process of reproductive functions which is required for successful pregnancy and affected by maternal psychological stress. It was observed that exposure to psychological stress inhibits the implantation in pregnant stressed mice. In addition, the activity and expression of SOD1 and SOD2 was upregulated during implantation window in stressed mice. Significant reduction in progesterone and increased estradiol level was also observed in stressed pregnant mice. newlineLater, the mice were adrenalectomized (ADX) to determine the in
Appears in Departments:Institute of Pharmacy

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04_declaration.pdf187.11 kBAdobe PDFView/Open
05_acknowledgement.pdf43.36 kBAdobe PDFView/Open
06_contents.pdf113.42 kBAdobe PDFView/Open
07_list of tables.pdf196.79 kBAdobe PDFView/Open
08_list of figures.pdf215.88 kBAdobe PDFView/Open
09_abbreviations.pdf96.67 kBAdobe PDFView/Open
10_chapter1.pdf627.02 kBAdobe PDFView/Open
11_chapter2.pdf240.3 kBAdobe PDFView/Open
12_chapter3.pdf388.35 kBAdobe PDFView/Open
13_chapter4.pdf1.71 MBAdobe PDFView/Open
14_summary.pdf223.93 kBAdobe PDFView/Open
15_bibliography.pdf432.95 kBAdobe PDFView/Open
16_publications.pdf266.31 kBAdobe PDFView/Open
1_title.pdf31.73 kBAdobe PDFView/Open
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