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Title: Structure-function correlations in antibacterial and cytolytic peptides: studies on Seminalplasmin : Seminalplasmin-derived and related synthetic peptides
Researcher: Sitaram, N
Guide(s): Nagaraj, R
Sitaram, S
Keywords: Cellular and molecular biology
Upload Date: 3-Mar-2014
University: Jawaharlal Nehru University
Completed Date: 1993
Abstract: One of the important aspects of protein chemistry is to relate activity to structure or newlineestablish structure-function relationships. During the past fifteen years, several proteins and newlinepeptides that are potent antibacterial and/or cytolytic agents have been purified and sequenced newlinefrom various organisms all along the evolutionary scale. One of the structural motifs which has newlinebeen identified to be important for the biological activities of several of these newlineantibacterial/cytolytic peptides is either an amphiphilic a-helical or amphiphilic p-structure. newlineHowever, factors that determine the specificity of antibacterial and hemolytic activities are not newlinewell understood. newlineThe investigations described in this thesis have been directed towards understanding the newlinestructure-function correlations in seminalplasmin (SPLN), an antimicrobial peptide present newlinein bovine seminalplasma. newlineThe thesis is divided into seven Chapters and the contents of the Chapters are briefly newlineoutlined below. newlineIn the introductory chapter - Chapter1- some of the well studied antimicrobial and newlinehemolytic peptides are reviewed. Those peptides which are covered include antimicrobial newlinepeptides from phagocyte granules, amphibian skin, insects, horseshoe crabs and hemolytic newlinepeptides like melittin, mastoparan, bombolitins and 8-toxin. Earlier work on seminalplasmin newline(SPLN) is also reviewed in this Chapter. newlineChapter 2 is devoted to the determination of the sequence of SPLN as well as its newlinetheoretical analysis. This Chapter is divided into two Sections, A and B. Section A reviews the newlinecurrent methods used in protein sequencing as well as methodsused for predicting secondary newline(vi) newlinestructure from the sequence .. In Section B purification of SPLN by HPLC and its sequencing is newlinedescribed. The Section also describes the methods developed for separating and identifying PTH newlineamino acids which is a pre-requisite for any sequencing work.
Pagination: iv, 185p.
Appears in Departments:Centre for Cellular and Molecular Biology

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01_title.pdfAttached File23.16 kBAdobe PDFView/Open
02_certificate.pdf19.59 kBAdobe PDFView/Open
03_acknowledgements.pdf63.43 kBAdobe PDFView/Open
04_contents.pdf47.32 kBAdobe PDFView/Open
05_abbrevations.pdf51.15 kBAdobe PDFView/Open
06_abstract.pdf116.52 kBAdobe PDFView/Open
07_list of publications.pdf37.86 kBAdobe PDFView/Open
08_chapter 1.pdf703.26 kBAdobe PDFView/Open
09_chapter 2.pdf1.02 MBAdobe PDFView/Open
10_chapter 3.pdf681.19 kBAdobe PDFView/Open
11_chapter 4.pdf647.56 kBAdobe PDFView/Open
12_chapter 5.pdf879.49 kBAdobe PDFView/Open
13_chapter 6.pdf602.76 kBAdobe PDFView/Open
14_chapter 7.pdf237.51 kBAdobe PDFView/Open
15_references.pdf312.32 kBAdobe PDFView/Open

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