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Title: Effect of some plant extract on non enzymatic glycosylation of IgG under in-vitro conditions
Researcher: Yadav, Dinesh Prasad
Guide(s): Singh, B
Chhipa, R C
Keywords: Biochemistry
Medicinal plants
Upload Date: 25-Feb-2014
University: Suresh Gyan Vihar University
Completed Date: August, 2012
Abstract: Medicinal plants are used in rural India to treat diabetes due to their traditional acceptability and availability. The plants are used as a crude extracts or as mixtures to treat diabetes. Plant extracts have their own importance and now being studied extensively due to having little or no side effects. Protein glycation takes place when elevated levels of reduced sugars react with amino groups in proteins, reaction known as Maillard reaction. If this process continues, it will lead to the formation of complex, often unstable, irreversible and reactive compounds “AGEs”, a process that may take weeks or even months to accomplish. The present study demonstrates that presence of AGEs-induced IgG damage in type 1, type 2 diabetes and hyperlipidemic patients. The study further purposes that in addition to IgG in serum concentration the quality of IgG molecule may not only be a crucial feature affecting its immunologic effects but also a risk factor as AGEs damage in diabetic and hyperlipidemic patients. The present study also provided evidence to suggest that the glycation of isolated IgG from diabetes and hyperlipidemic patients lead to alteration in the conformational as well as the biological properties of IgG. Further in present study Eulophia campestris and Eulophia nuda was selected and used to check the Maillard reaction inhibitory activity. Different combinations of glucose, protein and plant extracts (Eulophia campestris and Eulophia nuda) were made under in vitro conditions and their activity were monitored with trichloro acetic acid treatment method at 350 nm. Maillard reaction products/ AGEs were more with high glucose and high protein concentration and these were decreased by highest concentrationof Eulophia campestris and Eulophia nuda extract i.e. 30 mg/mL or 300 μL. Lower concentrations of plant extract produced either no or least response against Maillard reaction.
Pagination: 224p.
Appears in Departments:Department of Science

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01_title.pdf22.78 kBAdobe PDFView/Open
02_certificate.pdf132.4 kBAdobe PDFView/Open
03_acknowlegement.pdf6.63 kBAdobe PDFView/Open
04_abbreviations.pdf78.64 kBAdobe PDFView/Open
05_abstract.pdf76.6 kBAdobe PDFView/Open
06_content.pdf48.04 kBAdobe PDFView/Open
07_list of figure.pdf87.41 kBAdobe PDFView/Open
08_list of table.pdf15.11 kBAdobe PDFView/Open
09_chapter 1.pdf35 kBAdobe PDFView/Open
10_chapter 2.pdf1.82 MBAdobe PDFView/Open
11_chapter 3.pdf78.51 kBAdobe PDFView/Open
12_chapter 4.pdf400.98 kBAdobe PDFView/Open
13_chapter 5.pdf608.49 kBAdobe PDFView/Open
14_chapter 6.pdf101.72 kBAdobe PDFView/Open
15_chapter 7.pdf95.84 kBAdobe PDFView/Open
16_annexures.pdfAttached File397.2 kBAdobe PDFView/Open
17_publications.pdf104.34 kBAdobe PDFView/Open

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