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Title: Profiling of phenolic compound through UPLC-MS/MS and study on pharmaceutical properties of merremia emarginata ( Burm. F.)
Researcher: Rameshkumar, A
Guide(s): Sivasudha, T
Keywords: Environmental Biotechnology
merremia emarginata
Upload Date: 18-Jun-2013
University: Bharathidasan University
Completed Date: 2011
Abstract: Medicinal plants and other plant based food products are reported to be good source of natural antioxidants. The secondary metabolites such as phenolic compounds (phenolic acids and flavonoids etc) and other biomolecules such as amines, pigments, vitamins, steroids and alkaloids are the important antioxidants present in the plant based foods. Merremia emarginata (Burm. f.) synonym Ipomoea reniformis belongs to family Convolvulaceae is prevalent throughout India. This is uncultivated food crop used by poor people in India as green leaf vegetable. In Indian traditional medicine system, M. emarginata is used as deobstruent, diuretic, for rheumatism, neuralgia, cough and headache. Though, this plant has been used for treating many ailments for decades in Siddha and Ayurveda, but study of their pharmacological effects and the compounds responsible for their therapeutic property are yet to be identified. This thesis describes analytical methods for identification of the main constituents in the leaves of M. emarginata and its pharmacological uses. The preliminary phytochemical screening of aqueous leaf extract of M. emarginata indicates the presence of alkaloids, steroids, saponin, phenolics, flavonoids, reducing sugars and terpenoids. The in vitro antioxidant activity of M. emarginata leaf extract indicates the plant has potential to act as significant source of antioxidants. The antimicrobial activity of M. emarginata with quantum dots (QDs) was successfully studied and could be used for the pathogen reduction and detection. The phenolic compounds present in the M. emarginata were identified using UPLC MS/MS analysis. Phenolic compounds profiling shows 26 known and 19 unknown compounds in M. emarginata. Further, the diuretic property of M. emarginata was analysed in female Wistar rats. The polyphenolics (Chlorogenic acid) byproduct hippuric acid was successfully analysed in urine sample using RP-HPLC, it may act as polyphenolics induced diuretic marker.
Pagination: 100p.
Appears in Departments:Department of Environmental Biotechnology

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01_title.pdfAttached File124.99 kBAdobe PDFView/Open
02_certificate.pdf120.65 kBAdobe PDFView/Open
03_declaration.pdf108.02 kBAdobe PDFView/Open
04_dedication.pdf110.6 kBAdobe PDFView/Open
05_acknowledgements.pdf121.41 kBAdobe PDFView/Open
06_abstract.pdf85.18 kBAdobe PDFView/Open
07_abbreviations.pdf99.03 kBAdobe PDFView/Open
08_contents.pdf83.8 kBAdobe PDFView/Open
09_list of figures.pdf142.03 kBAdobe PDFView/Open
10_list of tables.pdf357.27 kBAdobe PDFView/Open
11_introduction.pdf348.97 kBAdobe PDFView/Open
12_chapter 1.pdf303.02 kBAdobe PDFView/Open
13_chapter 2.pdf828.45 kBAdobe PDFView/Open
14_chapter 3.pdf301.05 kBAdobe PDFView/Open
15_chapter 4.pdf1.16 MBAdobe PDFView/Open
16_conclusion.pdf136.53 kBAdobe PDFView/Open
17_references.pdf211.1 kBAdobe PDFView/Open

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