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Title: Antibody studies and bacterial drug resistance in typhoid fever
Researcher: Medhulika, Unnikishnan P
Guide(s): Harish, B N
Upload Date: 3-Nov-2010
University: Pondicherry University
Completed Date: May 2006
Abstract: Typhoid fever is a major cause of mohidity and mortality in the developing world. The causative organism, Salmonella Typhi, has developed resistance to many of the antibiotics used in freatment. It is essential to monitor the development of antibiotic resistance in order to formulate effective treatment policies. Typhoid fever is also associated with life threatening complications like ileal perforations, the pathogenesis of which is poorly understood. Aim and Objectives: The aims of this study were twofold-I) To look at antibiotic resistance in S. Typhi in general and fluoroquinolonc resistance in particular, and 2) To investigate the antibody profile in typhoid perforation. Materials and Methods This study included isolates of S.Typhi and serum samples from palicnts with signs and symptoms of typhoid fever, Isolation and identification followed standard pro~ocols. Antibiotic susceptibility was determined by Kirby- Bauer disc diffision method for a panel of antibiotics. The MIC was determined by both agar dilution and broth microdilution for ampicillin, chloramphenicol, ciprofloxacin and ceftriaxone and interpreted in accordance with NCCLS guidelines. Envelope profiling and PCR-RFLP were done for 30 selected isolates. PCR w a designed and performed for the amplification of 4 sequences located in the genes gyrA, morA, marl?, and rnarR. For antibody studies, 3 groups of samples were analysed- Group I, from patients with typhoid iled perforations. Group 11, from patients with uncomplicated typhoid fever md Gmup 111, consisting of healthy individuals or patienb with non-enteric infections. Antibodies were detected in these sera by immunoblotting using Whole Cell Rotein (WCP) extract, and also individually lor the H, LPS and OMP antigens. Results and d i d o n : During the 35 months of this study, 177 isolates of S.Typhi were obtained. Multidmg resistance was seen to decline over this period from 568 to 7%. In conhst to multidrug resistance, reduced ciprofloxacin susceptibility was seen in a majority of the isolates- 159, of which 157 were also nalidixic acid resistant. There was a good cornlation hetween nalidixic acid resistance and reduced ciprofloxacin susceptibility.
Pagination: 258p.
Appears in Departments:Jawaharlal Institute of Postgraduate Medical Education and Research

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01_title.pdfAttached File36.23 kBAdobe PDFView/Open
02_certificate.pdf38.48 kBAdobe PDFView/Open
03_declaration.pdf33.01 kBAdobe PDFView/Open
04_acknowledgement.pdf201.52 kBAdobe PDFView/Open
05_abstract.pdf119.98 kBAdobe PDFView/Open
06_index.pdf87.98 kBAdobe PDFView/Open
07_list of table.pdf32.43 kBAdobe PDFView/Open
08_list of figure.pdf49.1 kBAdobe PDFView/Open
09_abbreviation.pdf59.55 kBAdobe PDFView/Open
10_chapter 1.pdf166.27 kBAdobe PDFView/Open
11_chapter 2.pdf38.63 kBAdobe PDFView/Open
12_chapter 3.pdf3.8 MBAdobe PDFView/Open
13_chapter 4.pdf551.42 kBAdobe PDFView/Open
14_chapter 5.pdf944.05 kBAdobe PDFView/Open
15_chapter 6.pdf1.63 MBAdobe PDFView/Open
16_summary.pdf159.17 kBAdobe PDFView/Open
17_conclusion.pdf105.32 kBAdobe PDFView/Open
18_references.pdf2.17 MBAdobe PDFView/Open
19_appendices.pdf1.16 MBAdobe PDFView/Open

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