Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/9032
Title: Molecular analysis of cytokine polymorphism in conjunction with the study of immune response to common recall antigens, superantigens and xenobiotic compounds in autoimmune skin diseases
Researcher: Sajad Ahmad Dar
Guide(s): Shukla, Das
Ramachandran, V G
Bhattacharya, S N
Banerjee, B D
Keywords: Microbiology
Upload Date: 21-May-2013
University: University of Delhi
Completed Date: 2012
Abstract: Autoimmune diseases in susceptible individuals depend largely on multiple factors the genetic makeup, environmental antigen challenge and the immunological status of the afflicted. There is a paucity of knowledge on the risk posed by various environmental toxins, microbial antigen or superantigen stimulants which may modify the clinical expression of the autoimmune skin diseases and the response of the patient to treatment strategies. The participation of cytokines in the induction and effector phases of the immune and inflammatory response shows that their polymorphism may be playing a critical role in the development of autoimmune diseases. Objectives: Present study is an attempt to elucidate the role of various microbial antigens or superantigens (sAgs) produced by certain pathogens, and organochlorine pesticides (OCPs) in altering effector T cells and cytokine expression in patients of three autoimmune skin diseases viz., pemphigus, systemic sclerosis (SSc) and systemic lupus erythematosus (SLE). Efforts were also made to investigate single nucleotide polymorphism of an array of cytokines at DNA/genomic level to enhance our understanding of their role in disease progression in these autoimmune skin diseases. Methods: Fifty three patients diagnosed to have autoimmune skin disease pemphigus (20), systemic sclerosis (20) and systemic lupus erythematosus (13) were enrolled in the study. Peripheral venous blood (~10 ml) was collected aseptically from each patient and used for isolation of peripheral blood mononuclear cells (PBMCs), pesticide residue quantification and DNA extraction. Six months after induction of clinical remission by immunosupressives/steroids, repeat blood samples were collected from each patient for PBMCs isolation. Healthy age-matched volunteers were also enrolled for comparisons. PBMCs were stimulated in-vitro with different concentrations of microbial antigens or sAgs which included Staphylococcal enterotoxin B (SEB), Streptococcal pyrogenic exotoxin A (SPEA) and Cytomegalovirus (CMV)
Pagination: 238p.
URI: http://hdl.handle.net/10603/9032
Appears in Departments:Dept. of Microbiology

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02_certificate.pdf78.59 kBAdobe PDFView/Open
03_declaration.pdf71.53 kBAdobe PDFView/Open
04_acknowledgements.pdf126.2 kBAdobe PDFView/Open
05_contents.pdf95.27 kBAdobe PDFView/Open
06_list of tables.pdf84.03 kBAdobe PDFView/Open
07_list of figures.pdf90 kBAdobe PDFView/Open
08_list of abbreviations.pdf83.76 kBAdobe PDFView/Open
09_dedication.pdf22.37 kBAdobe PDFView/Open
10_over view.pdf103.07 kBAdobe PDFView/Open
11_chapter 1.pdf135.91 kBAdobe PDFView/Open
12_chapter 2.pdf842.34 kBAdobe PDFView/Open
13_chapter 3.pdf12.96 MBAdobe PDFView/Open
14_chapter 4.pdf286.01 kBAdobe PDFView/Open
15_references.pdf505.56 kBAdobe PDFView/Open
16_publications.pdf273.34 kBAdobe PDFView/Open
17_abstract.pdf167.43 kBAdobe PDFView/Open


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