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Title: Sequence characterization and expression analysis of toll-like receptor (TLRs) genes in Indian goat (Capra hircus)
Researcher: Goyal, Shubham
Guide(s): n.d.
Keywords: Bio Technology
Indian goat
Upload Date: 14-May-2013
University: Kurukshetra University
Completed Date: 2012
Abstract: Toll-like receptors (TLRs) are the membrane bound proteins, expressed on the cells mainly involved in innate and adaptive immunity. Upon recognition of different microbial products i.e. pathogen-associated molecular patterns (PAMPs) that are expressed by infectious agents, they initiate a signalling pathway to activate immune response and mediate the production of cytokines necessary for the development of effective immunity. Till date 13 TLRs have been discovered in mammals, out of which 10 have been well indentified and characterized in human, mouse, cattle, buffalo, sheep, chicken and pig. Though in goat sequencing of all the ten TLR genes has been reported but further characterization of these genes for polymorphism, expression and structure prediction has not been done so far. Therefore, looking at the importance of goat species to our agrarian economy and role on TLR genes in disease resistance, the present study was undertaken to characterize the TLR4, 7 and 8 genes of goat through sequence analysis, SNP detection, tissue distribution and characterization of expressed proteins. RNA was isolated from different tissues of goat and the DNA was extracted from the blood samples of different goat breeds, collected from their native breeding tracts. The isolated RNA was reverse transcribed and amplified with the help of gene specific primers for goat TLR4 and TLR8. The amplified products were ligated and transformed, the positive recombinant clones were sequenced and the raw sequence was further submitted to GenBank and the comparative analysis was done. TLR4 gene sequence showed maximum homology of approximately 99% with sheep both at amino acid and nucleotide levels as expected in comparison to other livestock species, the domain structure analysis by SMART online software also revealed the maximum structural homology of goat TLR4 with sheep. The secondary structure analysis also showed the same kind of results.
Pagination: 132p.
Appears in Departments:Department of Bio-Technology

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01_title.pdfAttached File112.23 kBAdobe PDFView/Open
02_contents.pdf61.19 kBAdobe PDFView/Open
03_abbreviations.pdf120.64 kBAdobe PDFView/Open
04_abstract.pdf79.27 kBAdobe PDFView/Open
05_chapter 1.pdf164.41 kBAdobe PDFView/Open
06_chapter 2.pdf631.72 kBAdobe PDFView/Open
07_chapter 3.pdf460.36 kBAdobe PDFView/Open
08_chapter 4.pdf2.58 MBAdobe PDFView/Open
09_chapter 5.pdf259.99 kBAdobe PDFView/Open
10_chapter 6.pdf191.71 kBAdobe PDFView/Open
11_references.pdf326.89 kBAdobe PDFView/Open
12_appendix.pdf101.28 kBAdobe PDFView/Open

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