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Title: Preparation of amperometric glycated hemoglobin biosensor based on zinc oxide nanoparticles fructosyl amino acid oxidase conjugate
Researcher: Sheetal Chawla
Guide(s): Pundir, C S
Keywords: Biochemistry
Fructosyl amino acid oxidase
Upload Date: 25-Apr-2013
University: Maharshi Dayanand University
Completed Date: 2012
Abstract: Measurement of HbA1c level in blood provides long-term glucose level in diabetic patients without influence of short-term fluctuations. The existing methods for HbA1C determination including biosensors suffer from insufficient sensitivity, detection limit, response time and storage stability. These problems were overcome in the present biosensor. A method is described for construction of an amperometric glycated hemoglobin (HbA1C) biosensor by immobilizing a fructosyl amino acid oxidase (FAO) onto ZnONPs/PPy hybrid film deposited on Au electrode and using it as working electrode, Ag/AgCl as reference electrode and Pt as auxillary electrode. The whole blood samples were hemolyzed and digested by protease before measuring their HbA1C level by the biosensor. The enzyme electrode detected FV as low as 50 and#61549;M at a signal to noise ratio of 3 within 2 s at +0.27 V vs. Ag/AgCl, pH 7.0 and 35oC with a linear working range, 0.1 to 3.0 mM for fructosyl valine (FV) and sensitivity of 38.42 and#61549;A mM-1. The electrode showed only 30% loss of its initial response over a period of 160 days, when stored at 4oC. Biosensor measured HbA1C in whole blood of apparently healthy and diabetic patients and found to be in the range, 4.0-5.6% and 5.7-12.0% respectively. The use of FAO/ZnONPs/PPy/Au electrode resulted into improved amperometric glycated hemoglobin biosensor.
Pagination: 93p.
Appears in Departments:Department of Bio-Chemistry

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01_title.pdfAttached File78.99 kBAdobe PDFView/Open
02_dedication.pdf115.01 kBAdobe PDFView/Open
03_declaration.pdf98.81 kBAdobe PDFView/Open
04_acknowledgements.pdf55.92 kBAdobe PDFView/Open
05_abbreviations.pdf118.57 kBAdobe PDFView/Open
06_abstract.pdf30.62 kBAdobe PDFView/Open
07_list of figures.pdf155.46 kBAdobe PDFView/Open
08_list of publications.pdf57.82 kBAdobe PDFView/Open
09_list of research publications.pdf6.51 kBAdobe PDFView/Open
10_contents.pdf327.02 kBAdobe PDFView/Open
11_chapter 1.pdf190.84 kBAdobe PDFView/Open
12_chapter 2.pdf1.04 MBAdobe PDFView/Open
13_chapter 3.pdf292.83 kBAdobe PDFView/Open
14_chapter 4.pdf1.01 MBAdobe PDFView/Open
15_chapter 5.pdf77.79 kBAdobe PDFView/Open
16_bibliography.pdf313.76 kBAdobe PDFView/Open
17_appendix.pdf779.84 kBAdobe PDFView/Open

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