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Title: Reproductive biology and tissue culturestudies in "Winged Bean" [Psophocarpus tetragonolobus (L.)DC]
Researcher: Koshy, Eapen P
Guide(s): John, Philip
Keywords: Biochemical analysis
Winged bean
Psophocarpus tetragonolobus
Tissue culture
Thin cell layer explants
Left cotyledon
Right cotyledon
Reproductive biology
Protein profile
Upload Date: 27-Feb-2013
University: Mahatma Gandhi University
Completed Date: March 2007
Abstract: Psophocarpus tetragonolobus (L.) DC., a potential backyard crop, has immense agricultural possibilities. The present study describes the floral biology of the plant and proposes successful micropropagation protocols. A detailed description of the plant was prepared along with a diagrammatic sketch. The morphology and ultrastructure of the reproductive organs were studied. Cytochemical analysis of the stigma reveals the localization of starch, lipids and proteins at various developmental stages. Thin cell layer nodal and stem explants gave maximum shoot formation, while whole tissue explants were inferior. The right cotyledon was superior to the left cotyledon in shoot regeneration. A three stage culture regime was necessary for successful callus regeneration induction, maintenance and regeneration stages. BAP + GA3 medium induced shoot elongation. Differentiated shoots were rooted to get healthy plantlets. Somatic embryos were initiated from leaf explants when 2,4-D induced callus was transferred to TDZ containing media and germinated in MS basal medium. About 90% survival of plants was obtained after hardening and acclimatization. RAPD and protein characterization revealed the clonal fidelity of the regenerants. Biochemical analysis of organogenetic and embryogenetic calluses revealed the possible role of metabolites and enzymes during differentiation and also their protein and pigment content.
Pagination: 134p.
Appears in Departments:Faculty of Science

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01_title.pdfAttached File38.17 kBAdobe PDFView/Open
02_declaration.pdf12.81 kBAdobe PDFView/Open
03_certificate.pdf23.86 kBAdobe PDFView/Open
04_dedication.pdf10.76 kBAdobe PDFView/Open
05_acknowledgements.pdf39.44 kBAdobe PDFView/Open
06_abstract.pdf13.63 kBAdobe PDFView/Open
07_preface.pdf15.49 kBAdobe PDFView/Open
08_contents.pdf35.75 kBAdobe PDFView/Open
09_list of tables.pdf13.96 kBAdobe PDFView/Open
10_list of figures.pdf13.03 kBAdobe PDFView/Open
11_abbreviations.pdf22.68 kBAdobe PDFView/Open
12_chapter 1.pdf159.97 kBAdobe PDFView/Open
13_chapter 2.pdf34.79 kBAdobe PDFView/Open
14_chapter 3.pdf214.84 kBAdobe PDFView/Open
15_chapter 4.pdf5.05 MBAdobe PDFView/Open
16_chapter 5.pdf193.3 kBAdobe PDFView/Open
17_chapter 6.pdf75.04 kBAdobe PDFView/Open
18_bibliography.pdf188.13 kBAdobe PDFView/Open
19_tables.pdf99.23 kBAdobe PDFView/Open
20_figures.pdf790.43 kBAdobe PDFView/Open
21_plates.pdf4.31 MBAdobe PDFView/Open

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