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Title: Molecular characterization and quantification Of toll like receptors in classical swine fever Virus infection in swine
Researcher: Veeresh B. Hanchinal
Guide(s): Dr.S.V.Hiremath
Keywords: Expression pattern and Classical swine fever virus (CSFV)
Swine, Real-time PCR,
Toll-like receptors (TLR)
University: KLE University
Completed Date: 01/06/2014
Abstract: Background XX ABSTRACT Classical swine fever (CSF) is one of the major economically important diseases of swine recognized by World Organization for Animal Health (WOAH/OIE). Auxiliary profession needs little investment in infrastructure and scientific approach results in a profitable outcome for the farmers. Majority of the pig farming in India is by the Northeastern states which constitutes to 28% of the total pig population in India. Classical swine fever (CSF), a Pestiviral (Classical swine fever virus, CSFV) disease has been a malady in the piggery industry affecting a large population and ending up wiping the total drove of the pigs. Objectives 1. Identification of TLRs in tissue of swine naturally infected with CSFV. 2. Quantification of the TLRs in the tissue collected. 3. Sequencing of the identified TLRs. 4. Study of the expression of TLRs in PK-15 cell line infected with CSFV virus. Methodology Using real-time PCR polymerase chain reaction (RT-PCR). Relative quantification was done by comparative CT method using and#946;-actin as the reference gene. Results Phylogenetic analysis of CFSV based on 5and#8223;UTR region and NS5B nucleotide sequence have showed that there are at least two subgroups (1.1 and 2.2) of CSFV present in India. The results showed that TLR1 TLR10 was expressed and detected at different levels in all tested swine tissues. The SYBR green real time PCR in infected tissue revealed elevated levels of TLR3, TLR7, TLR8 and TLR9. Expression levels of all selected TLRs was found to be at lower levels in the heart and high level of expression found in lymph node and liver.TLR7 gene was investigated and compared with that of gorilla, ovine, canine, bovine, macaques, goat, feline, human and mouse.TLR8 sequences in ovine, bovine, goat, human, mouse and gorilla. This revealed a steady state increase in the expression levels of TLR3, TLR7, TLR8 and TLR9. TLR7 and TLR8 further, showed high levels of expression when compared to other TLRs. Conclusion This study provides clear information concerning the health of s
Appears in Departments:Faculty of Interdisciplinary Studies

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