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Title: Effects of bisphenol a and octylphenol on the reproductive performance in albino mice
Researcher: Haloi, Akshay Kumar
Guide(s): Kalita, J C
Keywords: Albino
University: Gauhati University
Completed Date: 31/12/2005
Abstract: Environmental oestrogens are synthetic oestrogenic/anti-oestrogenic substances derived mainly from industrial sources such as detergents, paints, herbicides, pesticides and plastics and subsequently found in water and food. Initial observations in wildlife suggested that these chemicals caused adverse reproductive effects in animals. A major area of concern has been in relation to the possible link between the effects of environmental oestrogens and reported decline in sperm count and increasing reproductive disorders in men. The studies reported in this Thesis concentrate on the investigation of the reputed oestrogenic activity of two industrial chemicals Bisphenol-A and Octylphenol. Three different doses of each chemical i.e 50 mg, 75 mg and 100 mg/kg body weight/day were used in the studies. 17B-oestradiol was used as positive control. To determine the estrogenicity of the test chemicals the method described by Evans et al. (1941) were followed. Uterine protein and glycogen were estimated after the method described by Seifter et al. (1950) and Lowry et al. (1951) respectively. Total serum cholesterol was estimated after the method described by Zak (1957). DNA and RNA were extracted by the hydrolytic method of Schimdt Thannhauser-Schneider (1946) and was estimated following UV-spectrophotometer method described by Thimmaih, (1998). Histological studies were carried out by routine hematoxylin eosin according to the procedure described by Luna (1968). LD50 was determined using the method described by Omkar (1994) In the present studies, both the chemical have been shown to influence many aspects of mammalian reproductive processes via effects on the biochemical and histological alterations of reproductive system and alterations of sexual behavior. These changes were depended on the dose of exposure. Within treated groups, 17B-oestradiol gave the expected maximal response in all the experimental parameters. Between Bisphenol-A and Octylphenol, the highest activity was observed in the group receiving 100mg/kg body...
Appears in Departments:Department of Zoology

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02_certificate.pdf40 kBAdobe PDFView/Open
03_declaration.pdf20.98 kBAdobe PDFView/Open
04_abstract.pdf69.59 kBAdobe PDFView/Open
05_abbreviations.pdf62.33 kBAdobe PDFView/Open
06_publication.pdf32.13 kBAdobe PDFView/Open
07_content.pdf172.23 kBAdobe PDFView/Open
08_list of figures.pdf61.25 kBAdobe PDFView/Open
09_list of photographs.pdf37.35 kBAdobe PDFView/Open
10_list of tables.pdf37.92 kBAdobe PDFView/Open
11_acknowledgement.pdf117.65 kBAdobe PDFView/Open
12_chapter 1.pdf215.21 kBAdobe PDFView/Open
13_chapter 2.pdf2.69 MBAdobe PDFView/Open
14_chapter 3.pdf663.08 kBAdobe PDFView/Open
15_chapter 4.pdf7.31 MBAdobe PDFView/Open
16_discussion.pdf1.55 MBAdobe PDFView/Open
17_summary and conclusion.pdf251.42 kBAdobe PDFView/Open
18_figure studies.pdf55.78 kBAdobe PDFView/Open
19_references.pdf2.33 MBAdobe PDFView/Open

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