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Title: Biochemical Profile and Characterisation of Proteins in the Lens Extracted from Human Cataractous Subjects
Researcher: Sreeja S
Guide(s): Jeyanthi G P
Keywords: Cataractous lens
apparently normal cataract,
diabetic cataract
hypertensive cataract
lens crystalline proteins
University: Avinashilingam Deemed University For Women
Completed Date: 11.07.2014
Abstract: The present research work was designed to analyse the changes in soluble protein andinsoluble protein in the eye lens of the cataractous subjects To analyse any correlation between newlineselected biochemical parameters and insoluble protein content in eye lens To study the eye lens architecture in cataractous subjects and to study post translational changes in eye lens proteins newlineleading to cataract Subjects with and without clinical complications namely diabetes mellitus and hypertension were selected These subjects were then categorised into six groups of apparently newlinenormal cataract men ACM apparently normal cataract women ACW diabetic cataract men DCM diabetic cataract women DCW hypertensive cataract men HCM and hypertensive newlinecataract women HCW Changes in the status of soluble and insoluble proteins biochemical parameters namely enzymatic antioxidants non enzymatic antioxidants lipid peroxidation nitrite newlineprotein oxidation status enzymes of polyol pathway membrane bound enzymes basic biomolecules and glycoproteins were analysed Protein profiling in the eye lens extracted from newlinecataractous subjects were assessed The extent of DNA damage was assessed in six groups of cataractous lenses by comet assay Morphological and histopathological studies were carried out newlineto determine if there was any damage to the lens tissues Spectral analysis was carried out to identify the functional groups in the lens proteins of the cataractous subjects which might indicate newlineany post translational changes occurring in the cataractous lens The outcome of the study revealed that diabetic and hypertensive subjects seemed to develop cataract at an early age compared to newlineapparently normal cataractous subjects From this study diabetic cataractous subjects showed greater abnormalities during cataract development Major objectives The present research work was designed with the following objectives To analyse the changes in soluble protein and insoluble protein in the eye lens of the newlinecataractous subjects To analyse any correlation between selected biochemic
Appears in Departments:Department of Biochemistry, Biotechnology and Bioinformatics

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ssreeja_intro.pdf888.53 kBAdobe PDFView/Open

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