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Title: Cloning of bacterial cellulose gene into zymomonas mobilis for cellulosic ethanolproduction
Researcher: Thirumalai Vasan, P
Guide(s): John Vennison, S
Keywords: Biotechnology
Zymomonas mobilis
Carboxymethyl Cellulose
insect gut
Upload Date: 8-Nov-2012
University: Bharathidasan University
Completed Date: January 2011
Abstract: Five cellulolytic bacteria viz, Enterobacter cloacae, Pseudomonas aeruginosa, Klebsiella pneumoniae, Pseudomonas fluorescens and Proteus mirabilis were isolated from the gut of phytophagous insects. Cellulase gene from the above five bacteria was cloned separately into E. coli using pET20b(+) plasmid and the cellulase gene containing plasmids such as, pET-cel-Ec, pET-cel-Pa, pET-cel-Pm, pET-cel-Pf and pET-cel-Kp were developed and the cellulase genes were characterized by restriction analysis and DNA sequencing. The cloned cellulase genes were subcloned in pKT 230 plasmid, a stable vector of Zymomonas mobilis, an ethanol fermenting bacterium and the recombinant pKT-cel-Ec, pKT-cel Pa, pKT-cel-Pm, pKT-cel-Pf and pKT-cel- Kp were developed and transformed into Z. mobilis. These recombinant Z. mobilis strains expressing bacterial cellulase gene were used for ethanol production using carboxymethyl cellulose, 4% NaOH pretreated bagasse, 6% NaOH pretreated rice straw and 3% HCl pretreated coirpith as substrates. All the recombinant Z. mobilis strains were found to ferment ethanol from pretreated cellulosic substrates but the recombinant Z. mobilis strain harboring cellulase gene cloned from E. cloacae (pKT-cel-Ec) produced ethanol 12% (using glucose), 5.5% (using CMC), 4% (using 4% NaOH pretreated bagasse), 3.5% (using 6% NaOH pretreated rice straw) and 3% (using 3% HCl pretreated coirpith). The recombinant Z. mobilis strain could be improved further by simultaneous expression of additional cellulase genes and the strains could be used for industrial level ethanol production.
Pagination: 114p.
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File5.16 MBAdobe PDFView/Open
02_certificate.pdf5.16 MBAdobe PDFView/Open
03_declaration.pdf5.16 MBAdobe PDFView/Open
04_acknowledgements.pdf5.17 MBAdobe PDFView/Open
05_dedication.pdf5.16 MBAdobe PDFView/Open
06_preface.pdf5.2 MBAdobe PDFView/Open
07_contents.pdf5.17 MBAdobe PDFView/Open
08_list of tables.pdf5.18 MBAdobe PDFView/Open
09_abstract.pdf5.17 MBAdobe PDFView/Open
10_review of literature.pdf5.26 MBAdobe PDFView/Open
11_chapter 1.pdf5.27 MBAdobe PDFView/Open
12_chapter 2.pdf5.25 MBAdobe PDFView/Open
13_chapter 3.pdf5.22 MBAdobe PDFView/Open
14_chapter 4.pdf5.23 MBAdobe PDFView/Open
15_chapter 5.pdf5.22 MBAdobe PDFView/Open
16_summary.pdf5.17 MBAdobe PDFView/Open
17_references.pdf5.29 MBAdobe PDFView/Open
18_appendix.pdf5.18 MBAdobe PDFView/Open

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