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Title: Radioprotection by Coronopus didydmus (L.)- a free radical mechanistic and molecular approach
Researcher: Mudgal, Piya Paul
Guide(s): Unnikrishnan, M K
Naik, Nagappa Anantha
Keywords: Coronopus didymus
Upload Date: 5-Nov-2012
University: Manipal University
Completed Date: 03/05/2012
Abstract: Present study aimed at identifying the phytoconstituents responsible for radioprotection by Coronopus didymus (L.) (CD). Three flavonoids namely CD-C1, CD-C2 and CD-C3 were identified in the ethanolic extract of CD. An in-house method was also developed for obtaining a flavonoid mixture (CDFM). The antioxidant potential of the phytoconstituents and CDFM were investigated based on in vitro and in vivo radioprotection studies. CDFM effectively scavenged free radicals (R?), exhibiting maximum antioxidant potential compared to its constituent flavonoids. CD-C2 with two OH groups showed better antioxidant activity than CD-C3 with one OH group. At optimum concentrations, CDFM and CD-C1 (20 µg/ml) were most effective in scavenging reactive oxygen species in ?-irradiated V79 cells, followed by CD-C2 and CD-C3 (40 µg/ml). CDFM and the isolates, at their optimum concentrations, protected V79 cells from radiation-induced mutagenicity and genotoxicity. CDFM consistently showed better radioprotection, with a maximum increase in anti-apoptotic protein (Bcl-2), and a maximum decrease in the pro-apoptotic protein (cleaved caspase-3), than the individual flavonoids. CDFM (at 200 mg/kg, i.p., 30 min prior to ?-radiation) improved the overall survival in mice by 40%, possibly by protecting rapidly proliferating organs such as spleen and GIT. CDFM prevented hepatic lipid peroxidation which helped in preserving the endogenous antioxidant milieu consistent with in vitro and cell culture studies. The presence of CD-C2 (with a C4?-OH in its ring B) in CDFM, could be responsible for contributing to its anti-lipid peroxidation potential. In vivo radioprotection by CDFM could also be attributed to the increased absorption of its constituent flavonoid glycosides (CD-C2 and CD-C3) in cellular compartments. CD-C3, the major constituent of CDFM, protected mice against hematopoietic and hepatic damage, though not as efficiently as CDFM. However, CD-C3 failed to protect mice GIT from radiation damage.
Pagination: --
Appears in Departments:Manipal College of Pharmaceutical Sciences

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01_title.pdfAttached File164.52 kBAdobe PDFView/Open
02_certificate.pdf638.66 kBAdobe PDFView/Open
03_abstract.pdf163.04 kBAdobe PDFView/Open
04_declaration.pdf638.64 kBAdobe PDFView/Open
05_acknowledgement.pdf180.1 kBAdobe PDFView/Open
06_index page.pdf78.4 kBAdobe PDFView/Open
07_list of tables.pdf102.32 kBAdobe PDFView/Open
08_list of figures.pdf203.28 kBAdobe PDFView/Open
09_abbreviations.pdf244.32 kBAdobe PDFView/Open
10_chapter 1.pdf396.11 kBAdobe PDFView/Open
11_chapter 2.pdf1.25 MBAdobe PDFView/Open
12_chapter 3.pdf192.94 kBAdobe PDFView/Open
13_chapter 4.pdf669.54 kBAdobe PDFView/Open
14_chapter 5.pdf2.24 MBAdobe PDFView/Open
15_conclusion.pdf190.39 kBAdobe PDFView/Open
16_summary.pdf174.01 kBAdobe PDFView/Open
17_bibliography.pdf276.41 kBAdobe PDFView/Open

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