Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/4775
Title: Herbal lupeol as potent antidiabetic active principle on Sterptozotoin induced diabetic Wistar rats
Researcher: Lilly, V
Guide(s): Daisy, P
Keywords: plant
Infrared spectroscopy (IR)
diabetes
Kidney
Phytochemistry
Nanoparticles
Upload Date: 24-Sep-2012
University: Bharathidasan University
Completed Date: May, 2011
Abstract: Throughout human history, natural products have been used as remedies to cure or treat illnesses including diabetes mellitus. Lupeol is noteworthy compound to be focused. The present study was aimed to isolate lupeol from the shade dried leaves of Elephantopus scaber using bioassay guided fractionation. Petroleum ether, Hexane, ethyl acetate, methanol and aqueous solvents were used for the sequential preparation of the extracts. 250 mg/kg.b.wt of the extracts were administered orally to Streptozotocin (60 mg/kg.b.wt)-induced diabetic male albino Wister rats aged 9 weeks for 90 days to assess their effect on fasting plasma glucose level. Petroleum ether extract was identified as the best extract to isolate lupeol. The structure was elucidated using NMR studies. Lupeol was also administered orally at different doses (5,10,15,20 mg/kg.b.wt) for 60 days. It was found that plasma glucose was significantly (plt0.05) reduced in a dose dependent manner when compared to the normal. In addition, oral administration of extracts (250 mg/kg.b.wt) and lupeol (20 mg/kg.b.wt) significantly decreased serum glucose, HbA1c, triglycerides and total cholesterol at the same time markedly increased hemoglobin, tissue glycogen, HDL cholesterol, plasma insulin, C-peptide, serum protein and the expression of GLUT-4 mRNA and protein expression. Also the compounds restored the altered plasma enzyme, carbohydrate metabolizing enzymes, glycoproteins, 14C-glucose oxidation levels to near normal. Lupeol administration could result in the regeneration of and#946;-cells in the pancreatic cells. The 3D structure of lupeol docked with the insulin receptor by Discovery Studio 2.1 version (An Accelery?s product). The results show that lupeol can activate the receptor. These results confirm that lupeol possesses hypoglycemic activity. Hence, it could be considered for the development of potent antidiabetic drugs. In addition lupeol was loaded in Chitosan-Alginate(CS-ALG) nanoparticles to assess the possibility of CS-ALG nanoparticles as carriers for lupeol.
Pagination: 227p.
URI: http://hdl.handle.net/10603/4775
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File79.38 kBAdobe PDFView/Open
02_certificate.pdf49.4 kBAdobe PDFView/Open
03_declaration.pdf49.2 kBAdobe PDFView/Open
04_contents.pdf124.25 kBAdobe PDFView/Open
06_acknowledgement.pdf65.14 kBAdobe PDFView/Open
06_chapter 1.pdf180.86 kBAdobe PDFView/Open
07_chapter 2.pdf215.45 kBAdobe PDFView/Open
08_chapter 3.pdf345.82 kBAdobe PDFView/Open
09_chapter 4.pdf2.59 MBAdobe PDFView/Open
10_chapter 5.pdf211.99 kBAdobe PDFView/Open
11_summary.pdf104.75 kBAdobe PDFView/Open
12_appendix.pdf62.98 kBAdobe PDFView/Open
13_bibliography.pdf405.29 kBAdobe PDFView/Open
14_graphs, figures, photos and tables.pdf4.99 MBAdobe PDFView/Open
15_abstract in english.pdf71.51 kBAdobe PDFView/Open
16_abstract in tamil.pdf13.85 kBAdobe PDFView/Open


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