Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/4381
Title: Analysis of Thymine content in mRNAs and carbon content in proteins a computational approach
Researcher: Ananda Gopu, P
Guide(s): Rajendran, K
Jayaraj, V
Rajasekaran, E
Keywords: Biotechnology
Parkinson s disease
mRNAs
Carbon
Upload Date: 21-Aug-2012
University: Periyar Maniammai University
Completed Date: 16/03/ 2012
Abstract: Biological systems are information-rich systems. One of the major challenges today is solving the problem of genome-based diseases. There are hundreds of diseases that are reported to have their roots in the genome. These genomes underwent change during evolution, thus causing suffering to people. Though the impact is felt in all species from microorganisms to human, it is severe in mammals, particularly in human. In this context a detailed understanding of the genome, transcriptome and proteome is necessary to solve the problems of genome-based diseases. This research work focused on the role of thymine content in protein coding frames of mRNA sequences to analyze and identify the large hydrophobic residues (LHR) in protein sequences. The number and fraction of thymine in all six frames in each sequence of every species were calculated using a program written in C . This was the number and fraction of XTX in all six frames and the total number of base pairs were counted in each mRNA sequences and tabulated. Thymine is the only residue that gets changed to uracil in the base upon transcription. Protein coding frames of mRNA sequences prefers to have 27% XTX out of 100 codons such as (X= A, T, G or C). These XTXs code for large hydrophobic residues (LHRs) such as phenylalanine (F), isoleucine (I), leucine (L), methionine (M) and valine (V). Thymine in protein coding frames of mRNA sequences plays an important role in mRNA stability and activity. Therefore, the current approach tries to solve the main problems in mRNAs in the protein sequence instability. This makes the development of a tool for the genome-wide gene annotation. This research is also used to understand the role of defining thymine content in protein coding frames of mRNA sequences for protein synthesis i.e., hydrophobic interactions are the dominant force for stability in protein sequences. In human, a noticeable amount of mRNA sequences and its protein counter parts have got altered leading to malfunctioning of the proteins.
Pagination: 103p.
URI: http://hdl.handle.net/10603/4381
Appears in Departments:Department of Biotechnology

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02_certificate.pdf.pdf432.61 kBAdobe PDFView/Open
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04_acknowledgement.pdf.pdf421.35 kBAdobe PDFView/Open
05_abstract.pdf.pdf67.64 kBAdobe PDFView/Open
06_list of tables.pdf.pdf216.21 kBAdobe PDFView/Open
07_list of figures.pdf.pdf107.76 kBAdobe PDFView/Open
08_table of contents.pdf.pdf332.37 kBAdobe PDFView/Open
09_abbreviations.pdf.pdf127.49 kBAdobe PDFView/Open
10_chapter 1.pdf.pdf112.09 kBAdobe PDFView/Open
11_chapter 2.pdf.pdf884.78 kBAdobe PDFView/Open
12_chapter 3.pdf.pdf125.17 kBAdobe PDFView/Open
13_chapter 4.pdf.pdf204.65 kBAdobe PDFView/Open
14_chapter 5.pdf.pdf91.7 kBAdobe PDFView/Open
15_chapter 6.pdf.pdf190.67 kBAdobe PDFView/Open
16_chapter 7.pdf.pdf57.05 kBAdobe PDFView/Open
17_references.pdf.pdf96.56 kBAdobe PDFView/Open
18_appendix-i.pdf.pdf63.54 kBAdobe PDFView/Open
19_publication.pdf.pdf1.33 MBAdobe PDFView/Open


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