Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/4298
Title: Chromatographic separation of the Enantiomer of Chiral active pharmaceutical ingredients using molecular imprinting technology
Researcher: Balamurugan, K
Guide(s): Gokulakrishnan, K
Keywords: Chemistry
Molecular Imprinting Technology
Upload Date: 17-Aug-2012
University: Prist University
Completed Date: November, 2011
Abstract: Chromatographic separation of enantiomers is of prime importance in the development of active pharmaceutical ingredients. As a result of an intensive advancement in chiral separation technologies, several hundreds of chiral stationary phases (CSP) for liquid chromatography (LC) are now commercially available. But these columns are expensive and the analysis cost is also high. So efficient and cost effective molecularly imprinted column was developed and an efficient method was developed. In this work, molecular imprinting technology was employed to prepare a specific affinity sorbent for the resolution of drug enantiomers of Cathine, Phenylephrine, Pseudoephedrine, Ephedrine, Ritalinic acid and N-Methylephedrine. The molecularly imprinted polymer (MIP) was prepared by non-covalent molecular imprinting with either (+) or (-)-chiral drug molecule as a template on a polymer formed by copolymerization of methacrylic acid and ethylene glycol dimethacrylate (EGDMA)in presence of 2,2?-Azo-bisisobutyronitrile (AIBN) as the initiator in chloroform, at 5°C and under UV radiation. The resulting MIP was dried, ground and size reduced to uniform powder. The physical as well as the chemical properties of the polymeric particles so formed was carried out using Scanning Electron Microscopy (SEM), Brunauer, Emmett, Teller (BET) and particle size analysis. The powder was then slurry packed into the column and the template was washed off using suitable solvents. LC method was developed with polar organic mobile phases, and the influence of parameters such as temperature, the percentage of polar organic modifier, the nature and concentration of the acidic additive acetic acid in the mobile phase was first evaluated. The developed method was then fully validated as per International Conference on Harmonization (ICH) guidelines. The efficiency of the MIP column was compared with the commercially available CSP column and it was proved that the MIP column has greater resolution, precision, accuracy and better impurity detection.
Pagination: xxv, 271p.
URI: http://hdl.handle.net/10603/4298
Appears in Departments:Department of Chemistry

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01_title.pdfAttached File72 kBAdobe PDFView/Open
02_dedication.pdf249.63 kBAdobe PDFView/Open
03_acknowledgements.pdf80.42 kBAdobe PDFView/Open
04_declaration.pdf80.19 kBAdobe PDFView/Open
05_certificate.pdf80.55 kBAdobe PDFView/Open
06_abstract.pdf54.26 kBAdobe PDFView/Open
07_table of contents.pdf128.78 kBAdobe PDFView/Open
08_list of tables.pdf80.7 kBAdobe PDFView/Open
09_list of figures.pdf90.96 kBAdobe PDFView/Open
10_abbreviations.pdf64.38 kBAdobe PDFView/Open
11_chapter 1.pdf606.17 kBAdobe PDFView/Open
12_chapter 2.pdf411.17 kBAdobe PDFView/Open
13_chapter 3.pdf5.14 MBAdobe PDFView/Open
14_chapter 4.pdf3.23 MBAdobe PDFView/Open
15_chapter 5.pdf3.71 MBAdobe PDFView/Open
16_chapter 6.pdf3.25 MBAdobe PDFView/Open
17_chapter 7.pdf3.53 MBAdobe PDFView/Open
18_chapter 8.pdf3.23 MBAdobe PDFView/Open
19_chapter 9.pdf156.56 kBAdobe PDFView/Open
20_references.pdf151.34 kBAdobe PDFView/Open
21_list of publications.pdf806.03 kBAdobe PDFView/Open
22_vitae.pdf100.56 kBAdobe PDFView/Open


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