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Title: Identification characterization and comparative analysis of multigene family encoding major reactive oxygen species ros scavenging enzymes in Brassica sp
Researcher: Verma, Deepika
Guide(s): Kashmir Singh
Keywords: Anti-oxidant enzymes
Cloning and Expression
Expression Analysis
Reactive Oxygen Species
University: Panjab University
Completed Date: 2020
Abstract: Abiotic stresses in plants produce ROS, scavenged by anti-oxidant enzymes. Identification and characterization of SOD, CAT, APX, GPX and GR gene families in Brassica juncea, B. rapa and B. nigra was done using bioinformatics approaches, including chromosomal mapping, intron-exon organization, physico-chemical properties, sub-cellular location and 3D structure of proteins. Differentially expressed genes under abiotic-stresses in B. juncea and B. rapa were predicted using Trinityv2.03 and supported by the presence of cis-regulatory elements involved in abiotic stresses. Expression validation of candidate abiotic-stress responsive genes under drought and heat stresses was performed by qRT-PCR. Selected abioticstress responsive genes were cloned in expression vector pET-32a(+) and protein expression conditions were optimized. Enzyme and stability assays of purified recombinant BjuAFSD2a and BraAAPX2a proteins was done. newline
Pagination: 242p.
Appears in Departments:Department of Biotechnology

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02_certificate.pdf840.34 kBAdobe PDFView/Open
03_acknowledgement.pdf78.09 kBAdobe PDFView/Open
04_table of contents.pdf85.44 kBAdobe PDFView/Open
05_abbreviations.pdf104.32 kBAdobe PDFView/Open
06_chapter1.pdf436.63 kBAdobe PDFView/Open
07_chapter2.pdf667.17 kBAdobe PDFView/Open
08_chapter3.pdf703.07 kBAdobe PDFView/Open
09_chapter4.pdf7.32 MBAdobe PDFView/Open
10_chapter5.pdf433.39 kBAdobe PDFView/Open
11_chapter6.pdf327.74 kBAdobe PDFView/Open
12_bibliography.pdf655.83 kBAdobe PDFView/Open
13_appendices.pdf402.59 kBAdobe PDFView/Open
14_publications.pdf135.56 kBAdobe PDFView/Open
80_recommendation.pdf327.74 kBAdobe PDFView/Open

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