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Title: Characterization of Colored wheat with high Anthocyanin content
Researcher: Sharma, Saloni
Guide(s): Garg, Monika
Keywords: Agriculture biotechnology
Plant Science
University: Panjab University
Completed Date: 2020
Abstract: In this study, we developed and screened the advanced colored wheat lines at NABI, Mohali and characterized morphologically, biochemically, and nutritionally. They exhibited high anthocyanin content and verified to be having very good anti-oxidant capacity. UPLC chromatogram revealed that anthocyanin composition and peak pattern is not only dependent on donor genotype but also the background of recipient genotype. Nutrition parameters including major constituents, minor constituents, and processing quality parameters of advanced colored lines were similar to high yielding white wheat. Elemental analysis indicated significantly higher iron and zinc content in colored wheat lines. Further, animal cell culture-based assays of the anthocyanin enriched fractions from these lines suggested that they could attenuate lipopolysaccharide induced nitric oxide, IL-and#946; and TNF-and#945; production by the murine macrophage cell lines (RAW264.7). Colored wheat specifically black wheat followed by purple wheat shown to be a potent attenuator of HFD induced obesity and its co-morbidities along with in vivo strong antioxidant capacity. RNA-seq analysis also provides insights into the molecular mechanism behind the preventive effect of anthocyanin rich colored wheat. On the other hand, transcriptome analysis of colored wheat seeds provides evidence that F3H, a structural gene of the flavonoid pathway act as a controlling enzyme of anthocyanin accumulation in pigmented wheat. Therefore, our advanced colored wheat lines with high anthocyanin, iron, and zinc contents showed antioxidant, anti-inflammatory activity, and anti-obesity effects and possessed desirable features for product making and commercial utilization. newline
Pagination: x, 168p.
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File80.95 kBAdobe PDFView/Open
02_certificate.pdf271.3 kBAdobe PDFView/Open
03_acknowledgement.pdf65.42 kBAdobe PDFView/Open
04_contents.pdf90.51 kBAdobe PDFView/Open
05_list of tables.pdf154.71 kBAdobe PDFView/Open
06_list of figures.pdf175.08 kBAdobe PDFView/Open
07_list of abreviations.pdf180.47 kBAdobe PDFView/Open
08_chapter1.pdf233.21 kBAdobe PDFView/Open
09_chapter2.pdf2.51 MBAdobe PDFView/Open
10_chapter3.pdf641.91 kBAdobe PDFView/Open
11_chapter4.pdf3.91 MBAdobe PDFView/Open
12_chapter5.pdf154.32 kBAdobe PDFView/Open
13_summary.pdf123.23 kBAdobe PDFView/Open
14_biography.pdf465.99 kBAdobe PDFView/Open
15_annexure.pdf563.12 kBAdobe PDFView/Open
80_recommendation.pdf123.23 kBAdobe PDFView/Open

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