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Title: Molecular and genetics basis of amylase resistant starch variation in bread wheat Triticum aestivum L
Researcher: Mishra, Ankita
Guide(s): Roy, Joy K
Keywords: Bread Wheat
Genotyping by sequencing
High Amylose
QTL mapping
Resistant Starch
University: Panjab University
Completed Date: 2019
Abstract: The modern lifestyle and nutritionally poor food supply have raised the concern of obesity and health related issues. To address the later, this study aimed to enhance nutritional quality of bread wheat by altering amylose content. In order to generate variation for grain amylose/Resistant starch content in Indian wheat genotypes a mutant population was developed in background of C 306 . The mutant population had a variation of amylose content (3% to 75%). Two lines TAC 75 and TAC 6 having amylose content 64% and 7% respectively was used to study the molecular basis of trait. The gene expression analysis of starch pathway genes in these lines indicated the role of GBSSI and SBEII in mutants. The global transcriptome analyses further identified a number of differentially expressed genes that provide the candidate to study the regulation of starch biosynthesis. The high amylose trait was transferred to an Indian wheat variety, WH 1105 (high yielding variety) from TAC 75 . The advanced F2 and F3 populations were sequenced using GBS technology and QTL mapping was performed using 1,572 SNP markers. The QTL mapping identified 12 major effect QTLs. Identification of the QTL genes under the two stable QTLs identified 265 protein coding genes, including two genes GBSSI and Sucrose synthase SUS1 as the potential candidates. newlineAltogether, the mutant population, DEGs, and QTLs will facilitate the breeding and MAS for amylose content in Indian genotypes. The lines with high resistant starch content are good preventive measure for the diabetes and related health issues. newline newline
Pagination: 122p.
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File9.21 kBAdobe PDFView/Open
02_certificate.pdf131.93 kBAdobe PDFView/Open
03_acknowledgement.pdf59.37 kBAdobe PDFView/Open
04_contents.pdf15.25 kBAdobe PDFView/Open
05_list of figures.pdf203.35 kBAdobe PDFView/Open
06_list of tables.pdf165.81 kBAdobe PDFView/Open
07_abbreviation.pdf131.17 kBAdobe PDFView/Open
08_chapter1.pdf7.75 MBAdobe PDFView/Open
09_chapter2.pdf7.93 MBAdobe PDFView/Open
10_chapter3.pdf7.75 MBAdobe PDFView/Open
11_chapter4.pdf7.75 MBAdobe PDFView/Open
12_chapter5.pdf7.75 MBAdobe PDFView/Open
13_summary.pdf7.75 MBAdobe PDFView/Open
14_bibliography.pdf7.75 MBAdobe PDFView/Open
15_annexures.pdf7.75 MBAdobe PDFView/Open
80_recommendation.pdf7.75 MBAdobe PDFView/Open

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