Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/291963
Title: Probiotic Lipases Applications to Polymer Degradation Biofuel Synthesis and Greener Nanobiotechnology
Researcher: Khan Mohammed Imran
Guide(s): Jayati Ray Dutta
Keywords: Biochemistry and Molecular Biology
Biology and Biochemistry
Life Sciences
University: Birla Institute of Technology and Science
Completed Date: 2019
Abstract: Lipases play a very important role as natural biocatalysts of several biochemical reactions. There has been an increased demand for microbial lipases due to inability of plant and animal lipases to meet the global requirement. The current focus of the study was intended to explore a probiotic lipase obtained from Lactobacillus sp. for various applications such as polymer degradation, cell growth studies on degraded polymer film, biofuel production, nanoparticle synthesis, their characterization and nanobiotechnological applications. This probiotic lipase was isolated from Lactobacillus plantarum, Lactococcus brevis strains and their co-cultures. The production parameters were optimized by both classical, statistical methods previously established in our lab, followed by cloning and expression to further improve the yield. newlineIn the present study, three different lipases derived from Lactobacillus brevis, Lactobacillus plantarum and their co-culture have been utilized to explore their efficiency towards Poly-and#949;-caprolactone [PCL] degradation via enzyme pouring method. The effect of parameters like enzyme loading and degradation time had been studied to understand the efficiency of the enzymes used. Various characterization techniques such as thermogravimetric analysis (TGA), differential thermal analysis (DTA), scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) have been employed to study the enzymatic degradation and its possible mechanistic insight. The enzymatic degradation was found to be significantly influenced by the concentration, time of exposure and the type of the enzyme. Among the three enzymes screened in this study, the lipase from Lactobacillus plantarum was found to possess the maximum PCL degradation activity at a nominal loading of 5 mg/mL. Thermogravimetric analysis results showed that with increasing exposure time of enzyme, the thermal stability of the newlinev newlinePCL films decreased due to its degradation. The DTA analyses revealed that the enzyme preferentially degraded
Pagination: 187p.
URI: http://hdl.handle.net/10603/291963
Appears in Departments:Biological Science

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