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Title: Production Characterization and Immobilization of Thermoalkali Stable Xylanase from Geobacillus Thermodenitrificans X1 and its Application in Paper Pulp Bio Bleaching
Researcher: Verma, Ritika
Guide(s): Sudhir Kumar
Keywords: Bio-bleaching
Geobacillus thermodenitrificans
Life Sciences,Microbiology,Biotechnology and Applied Microbiology
University: Jaypee University of Information Technology, Solan
Completed Date: 2019
Abstract: In the current study, cellulase free thermo-alkali-stable xylanase was produced through a cost effective method; followed by its immobilization and application in paper pulp bio-bleaching. A potent thermophilic xylanolytic bacteria; identified as Geobacillus thermodenitrificans X1 (GTX1) was isolated from Tattapani hot spring soil. The process of xylanase production from GTX1 was optimized by two methods: one-factor-at-a-time method (OFAT) and response surface methodology (RSM). Highest xylanase titer of 18.5 U/mL was observed after optimization by OFAT, whereas 1.3-fold elevation in the titer (24and#8201;±and#8201;2 U/mL) was observed after RSM. For minimizing the production cost, commercial xylan was substituted by lignocellulosic residues. The xylanase titer achieved by utilizing lignocellulosic residues in the present study is highest in comparison to that, reported from other Geobacillus thermodenitrificans isolates. The crude GTX1 xylanase was further immobilized to form xylanase cross-linked enzyme aggregates (Xy-CLEAs). Various parameters influencing the preparation of Xy-CLEAs were optimized. GTX1 Xy-CLEAs rendered spherical shape with 202 nm diameter. After immobilization variation in the temperature and pH optima of xylanase was observed. Also, Xy-CLEAs rendered better stability at high temperature (70ºC) and alkaline pH (10) in comparison to free xylanase. The promising commercial potential of Xy-CLEAs was reflected through its reusability for six consecutive cycles with 53.5% retained xylanase activity and storage stability with an efficiency of 86% activity after being stored for 8 weeks at 4 ºC. Immobilization through preparation of Xy-CLEAs was observed to be advantageous in terms of enhanced functional and operational stability. The only loophole in the process was decreased xylanase activity after immobilization. Further, for exploring the potential of GTX1 xylanase as a bio-bleaching agent in the paper industry, crude xylanase was used. Bio-bleaching application of GTX1 xylanase was conducted in RandD.
Pagination: xx, 99p.
Appears in Departments:Department of Biotechnology

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