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Title: Biofortification of Indian banana for enhanced pro vitamin a PVA content
Researcher: Shivani
Guide(s): Tiwari , Siddharth
Keywords: Life Sciences,Microbiology,Biotechnology and Applied Microbiology
Pro-Vitamin A
Embryogenic Cell suspension
Agrobacterium-Mediated Transformation
University: Panjab University
Completed Date: 2019
Abstract: Biofortification of popular Indian banana cultivars, i. e. Grand Naine and Rasthali was done to increase the pro-vitamin A (PVA) content. The work was started with Queensland University of Technology (QUT), Australia under technology transfer project. The regeneration protocol was optimized for Grand Naine and Rasthali. The lower regeneration efficiency obtained in Grand Naine was enhanced from 68% to 81% by spraying 2, 4-diphenoxyacetic acid on immature male flower buds. In-silico analysis was done to identify the nine transcription (TF) families that play a crucial role in somatic embryogenesis (SE). The higher expression of Musa acuminata babyboom 2 (MaBBM2) and Musa acuminata wuschel 2 (MaWUS2) in embryogenic cells as compared to non-embryogenic cells of Grand Naine showed their promising role in banana SE. Agrobacterium tumefaciens-mediated transformation was optimized using the and#946;-glucouronidase (GUS) reporter gene for both the cultivars. Grand Naine embrogenic cell suspension (ECS) showed lower transformation efficiency as compared to Rasthali. The number of Grand Naine transformants increased from 46.70±2.50 plantlets/ml settled cell volume (SCV) to 72.30±7.50 plantlets/ml SCV using melatonin antioxidant. In the present study, two gene constructs received from the QUT, Australia containing Asupina Phytoene synthase 2a (APSY2a) under fruit-specific (1- aminocyclopropane-1- carboxylate oxidase: ACO) and constitutive (Ubiquitin) promoters were overexpressed in both cultivars. The highest PVA/and#946;-carotene equivalents (and#946;-CE) content obtained in ripe fruit pulp of Grand Naine was up to 34.40 and#956;g/g DW and up to 72.40 and#956;g/g DW in Rasthali fruit pulp with constitutive promoter using high performance liquid chromatography (HPLC). The selected promising lines will be considered for further evaluations. newline newline newline
Pagination: 117p.
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File80.52 kBAdobe PDFView/Open
02_certificate.pdf592.34 kBAdobe PDFView/Open
03_acknowledgement.pdf59.11 kBAdobe PDFView/Open
04_contents.pdf91.14 kBAdobe PDFView/Open
05_list of figures.pdf91.92 kBAdobe PDFView/Open
06_list of tables.pdf131.46 kBAdobe PDFView/Open
07_abbreviations.pdf275.29 kBAdobe PDFView/Open
08_chapter 1.pdf223.39 kBAdobe PDFView/Open
09_chapter 2.pdf840.66 kBAdobe PDFView/Open
10_chapter 3.pdf367.57 kBAdobe PDFView/Open
11_chapter 4.pdf1.63 MBAdobe PDFView/Open
12_chapter 5.pdf1.16 MBAdobe PDFView/Open
13_chapter 6.pdf2.55 MBAdobe PDFView/Open
14_summary.pdf162.76 kBAdobe PDFView/Open
15_references.pdf446.47 kBAdobe PDFView/Open
16_appendix.pdf3.7 MBAdobe PDFView/Open
17_list of publications and presentations.pdf159.3 kBAdobe PDFView/Open

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