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Title: Molecular and functional characterization of plant inositol pyrophosphate kinase
Researcher: Mandeep Kaur
Guide(s): Pandey, Ajay Kumar
Keywords: Dehydration
Drought Stress
Inositol Pyrophosphate Kinase
Life Sciences,Microbiology,Biotechnology and Applied Microbiology
University: Panjab University
Completed Date: 2019
Abstract: The inositol pyrophosphates(PPx-InsPs): InsP7 and InsP8 are novel signaling molecules possessing high- energy pyrophosphate bonds. These unique group of inositol metabolites are involved in multiple physiological processes including bioenergetics and phosphate homeostasis. Herein, we have discerned two VIP-like homologs from wheat: TaVIH1 and TaVIH2 possessing a dual-domain structure. Analysis of transcript abundance revealed that TaVIHs were differentially expressed across various tissue samples. Both VIH genes showed contrasting transcriptional changes under phosphate limiting conditions, which depicted their involvement in regulation of phosphate starvation response. Biochemical assays showed that recombinant wheat VIHs exhibit in vitro IP6 kinase activity. The GUS-reporter assays in homozygous transgenic lines of Arabidopsis displayed strong reporter activity for VIH2-promoter in response to Pi-starvation, dehydration and drought conditions. Overall, our results confirm the new functional link of inositol pyro-phosphate kinases with stress response in wheat.
Pagination: 100p.
Appears in Departments:Department of Biotechnology

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03_acknowledgements.pdf76.1 kBAdobe PDFView/Open
04_contents.pdf5.52 kBAdobe PDFView/Open
05_abbreviations.pdf156.23 kBAdobe PDFView/Open
06_list_of_figures.pdf167.93 kBAdobe PDFView/Open
07_list_of_tables.pdf71.68 kBAdobe PDFView/Open
08_chapter1.pdf426.1 kBAdobe PDFView/Open
09_chapter2.pdf655.02 kBAdobe PDFView/Open
10_chapter3.pdf752.96 kBAdobe PDFView/Open
11_chapter4.pdf1.63 MBAdobe PDFView/Open
12_chapter5.pdf1.46 MBAdobe PDFView/Open
13_summary.pdf130.38 kBAdobe PDFView/Open
14_bibliography.pdf383.88 kBAdobe PDFView/Open
15_publications.pdf194.53 kBAdobe PDFView/Open

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