Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/262029
Title: Persistence of Acinetobacter baumannii against antibiotics
Researcher: Amanjot Kaur
Guide(s): Capalash, Neena and Sharma, Prince
Keywords: Acinetobacter baumannii
Biofilm
Curcumin
Lactonase
Life Sciences,Microbiology,Biotechnology and Applied Microbiology
Persister Cells
University: Panjab University
Completed Date: 2019
Abstract: Persisters are phenotypic variants of normal susceptible bacterial populations that survive prolonged exposure to high doses of antibiotics and are responsible for relapse of infection. Acinetobacter baumannii formed highest percentage of persister cells against rifampicin followed by amikacin and least against colistin. In the present study, three different anti-persister approaches were employed to target the persister cells. First, curcumin synergistically increased reactive oxygen species (ROS) production in combination with colistin, reducing significantly the survival of persister cells. Second, exogenous supplementation of metabolites viz. glucose, fructose or citrate or osmolytes increased proton- motive force (PMF) that facilitated amikacin uptake leading to decreased newlinepersister cells survival. Third, quorum-quenching enzyme lactonase effectively reduced persister cells against antibiotics in biofilm formed in its presence. These strategies can be effectively employed as treatment options with anti-persister potential for the control of chronic and relapsing A. baumannii infections.
Pagination: 197p.
URI: http://hdl.handle.net/10603/262029
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File177.76 kBAdobe PDFView/Open
02_certificate.pdf335.55 kBAdobe PDFView/Open
03_acknowledgement.pdf36.33 kBAdobe PDFView/Open
04_abbreviations.pdf9.7 kBAdobe PDFView/Open
05_contents.pdf5.64 kBAdobe PDFView/Open
06_chapter 1.pdf93.59 kBAdobe PDFView/Open
07_chapter 2.pdf804.74 kBAdobe PDFView/Open
08_chapter 3.pdf531.68 kBAdobe PDFView/Open
09_chapter 4.pdf4.16 MBAdobe PDFView/Open
10_chapter 5.pdf308.72 kBAdobe PDFView/Open
11_chapter 6.pdf183.58 kBAdobe PDFView/Open
12_references.pdf329.4 kBAdobe PDFView/Open
13_annexure.pdf139.8 kBAdobe PDFView/Open


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