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Title: Molecular evaluation of water samples and water treatment protocols with special reference to Hepatitis A and Hepatitis E Viruses
Researcher: Verma, Vikram
Guide(s): Arankalle, Vidya A
Keywords: Water, Viruses, Viral diseases, Hepatitis A, Hepatitis E
Upload Date: 2-Sep-2011
University: University of Pune
Completed Date: December, 2006
Abstract: Waterborne viral diseases resulting from the consumption of contaminated drinking water, inadequate supply of water for personal hygiene and poor sanitation take heavy toll worldwide and developing countries are the major sufferers. Though presence of human enteric viruses has been demonstrated in water bodies as well as drinking water supplies throughout the world and several outbreaks of enteric viral diseases attributed to virologically contaminated water have been recorded, routine examination of water samples for the presence of enteric viruses is not carried out in both developed and developing countries. The main reason for not carrying out such an examination remains unavailability of a rapid, cost-effective and efficient standard procedure. The basic steps of the virological analysis of water are sampling, concentration, and specific virus detection. Concentration of water samples is a critical step, since the viruses may be present in such low numbers that it is necessary to use very large volumes and concentrate it up to a few milliliters. Recognizing the necessity of an assay for virological evaluation of water samples, this study undertook the task of developing an efficient, sensitive, quick and relatively inexpensive protocol for concentration of viruses from large amount of water samples followed by simultaneous detection of Hepatitis A Virus (HAV), Hepatitis E Virus (HEV), entero and rotaviruses. The efficacy of water supply system of Pune, India and the prevalence of enteric viruses in Mutha River flowing through Pune city was also assessed. Evaluation of point-of-use water purification devices for their efficiency to remove the virus from seeded water samples was another objective.
Pagination: 159p.
Appears in Departments:National Institute of Virology

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02_certificate.pdf59.45 kBAdobe PDFView/Open
03_declaration.pdf58.61 kBAdobe PDFView/Open
04_dedication.pdf20.49 kBAdobe PDFView/Open
05_acknowledgement.pdf62.64 kBAdobe PDFView/Open
06_abstract.pdf81.57 kBAdobe PDFView/Open
07_index.pdf71.85 kBAdobe PDFView/Open
08_list of abbreviations.pdf102.53 kBAdobe PDFView/Open
09_list of figures.pdf69.74 kBAdobe PDFView/Open
10_list of tables.pdf69.3 kBAdobe PDFView/Open
11_chapter 1.pdf256.57 kBAdobe PDFView/Open
12_chapter 2.pdf93.33 kBAdobe PDFView/Open
13_chapter 3.pdf498.83 kBAdobe PDFView/Open
14_chapter 4.pdf939.74 kBAdobe PDFView/Open
15_chapter 5.pdf147.53 kBAdobe PDFView/Open
16_chapter 6.pdf98.06 kBAdobe PDFView/Open
17_references.pdf218.02 kBAdobe PDFView/Open

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