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Title: Studies on evaluation and improvement in the bioleaching capabilities of bacterial isolates from mines
Researcher: Hosseinabadi, Daryoush Arabian
Guide(s): Gade, W N
Keywords: Biotechnology
Upload Date: 1-Sep-2011
University: University of Pune
Completed Date: 2009
Abstract: The term bioleaching refers to the conversion of an insoluble metal (usually a metal sulfide, e.g., CuS, NiS, and ZnS) into a soluble form (usually the metal sulfate, e.g., CuSO4, NiSO4, ZnSO4). When this happens, the metal is extracted into water; this process is called bioleaching (Torma. 1977). These processes are oxidative in nature and when microorganisms mediate these, they are termed as biooxidation. Here the recovery of a metal is enhanced by microbial decomposition of the mineral. In this process the metal being recovered remains in insoluble form. An example is the recovery of gold from arsenopyrite ores where the gold remains in the mineral after biooxidation and is extracted by cyanide in a subsequent steps. Hence the term bioleaching is clearly inappropriate when referring to gold recovery (although arsenic, iron, and sulfur are bioleached from the mineral). Biomining is a general term that may be used to refer to both the processes. Populations of leaching bacteria are found ubiquitously and may be isolated with little difficulty wherever oxidizable ore bodies are exposed to the surface. However, newly isolated bacterial populations cannot be expected to oxidize ores at very high rates. The challenge to the biotechnologist is to improve the rate of cell growth and ore oxidation by the microbes responsible for leaching. OBJECTIVES OF STUDY: 1- Isolation and characterization of native leaching microorganisms from ores 2- Optimization of metal extractions protocols from mine. 3- Strain improvement of bacteria by adaptation to higher metal concentrations so as to improve the metal extraction efficiency. 4- To evaluate the resistance level of isolated bacterial strains to various metals and to mechanism of resistance to zinc and lead at molecular level. 5- Developing suitable column bioreactors for bioleaching of Zinc and Lead at pilot scale. In the present study Iranian zinc and lead sulphied complex mine was selected for bioleaching propose.
Pagination: 237p.
Appears in Departments:Department of Biotechnology

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02_certificate.pdf12.03 kBAdobe PDFView/Open
03_declaration.pdf12.96 kBAdobe PDFView/Open
04_acknowledgement.pdf13.13 kBAdobe PDFView/Open
05_dedication.pdf12.83 kBAdobe PDFView/Open
06_abstract.pdf75.97 kBAdobe PDFView/Open
07_contents.pdf38.1 kBAdobe PDFView/Open
08_abbreviation.pdf20.01 kBAdobe PDFView/Open
09_chapter 1.pdf466.32 kBAdobe PDFView/Open
10_chapter 2.pdf370.5 kBAdobe PDFView/Open
11_chapter 3.pdf196.77 kBAdobe PDFView/Open
12_chapter 4.pdf384.91 kBAdobe PDFView/Open
13_chapter 5.pdf370.23 kBAdobe PDFView/Open
14_chapter 6.pdf88.69 kBAdobe PDFView/Open
15_chapter 7.pdf53.21 kBAdobe PDFView/Open
16_references.pdf229.18 kBAdobe PDFView/Open

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