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Title: Study on the mechanism of metal nano oxide induced stress on Type 2 Pneumocytes cultured in vitro
Researcher: Martin Ansie
Guide(s): Angshuman Sarkar
Keywords: Life Sciences,Biology and Biochemistry,Biochemistry and Molecular Biology
University: Birla Institute of Technology and Science
Completed Date: 2018
Abstract: We live in a world of exponential nano ingenuity. Exposure of engineered nanoparticles becomes inevitable and thus there is an increasing concern on nanomaterial safety and induced toxicity. Owing to small size and high surface area, nanoparticles are more penetrable and reactive with biological tissue than their bulk counterparts. Decades of research has shown nanotoxicity implements vastly different mechanisms and as such with any type of nanomaterial, it is important to understand the course of molecular events following their exposure. Metal oxide nanoparticles are the largest class of manufactured nanomaterials, owing to their wide range of application. The exposure is pronounced at industries and manufacturing facilities that discharge waste including aerosolized suspensions. A large part of atmospheric presentation as vehicular effluents also adds to environmental pollution. Such a widely encountered exposure can present risk of toxicity. Nanotoxicity from metal oxides has been documented to realize alterations in cellular morphology, DNA damage, internalization, modulation of protein synthesis and ultimately causes cellular death. Although thus far there is no comprehensive study that elucidates molecular mechanisms of toxicity induced by metal nano oxides in pulmonary tissue. Especially for alveolar cells that are highly susceptible to aerosolized nanoparticles as they form the first line of entry into the human body. newlineThis PhD thesis aimed to understand the mechanism of toxicity induced by metal nano oxides on alveolar type II cells. A systematic approach has been carried out with a less lethal (TiO2) and a highly lethal (ZnO) metal oxide nanoparticle in understanding the differences between cellular responses to their exposure. Alveolar type II cells, A549 cell line is outlined for the scope of this thesis, which are widely studied as in vitro models. This study has discovered some very novel findings in the field of nanotoxicology. These are involvement of cellular filopodia, HMGB1, Hsp70, cdc42, EMT a
Pagination: 206
Appears in Departments:Biological Science

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