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Title: Enhanced production of thrombinase: a therapeutic enzyme
Researcher: Naveena, B
Guide(s): Partha, N
Keywords: actinomycetes
Cardiovascular Diseases
Therapeutic Enzyme
Upload Date: 12-Sep-2014
University: Anna University
Completed Date: n.d.
Abstract: Production of various enzymes has been so far reported out using newlinedifferent sources such as plants animals and microbes Among them newlinethrombinase is one which possesses significant medical applications for the newlinetreatment of Cardiovascular Diseases CVDs caused by intravascular newlinethrombosis Though the enzyme has been so far reported by various sources newlinethe necessity of enzyme which would not cause any side effects is getting newlineprompted nowadays Hence marine microorganism has been used as a newlinesource of thrombinase production in this study newlineThrombinase producing actinomycetes were isolated from the newlinemarine water sample using fibrin plate assay The spore morphology of the newlinemarine isolate was analyzed by light and Scanning Electron Microscopy newlineSEM Further the marine isolate was identified and named as Streptomyces newlinevenezuelae ACT1 through biochemical tests 16srRNA analysis and newlinephylogenetic tree construction The gene sequence obtained thorugh 16srRNA newlineanalysis was published in National Centre for Biotechnology Information newlineNCBI and received the accession number as Streptomyces venezuelae ACT newline1 GenBank ID JQ039359 newlineThe effect of ultrasonication on biomass and thrombinase activity newlinewas investigated with respect to time The results revealed that both the cell newlineconcentration and the enzyme activity increased with the effect of newlineultrasonication The cell dry weight and the enzyme activity of the marine newlineisolate in ultrasonic treated medium were found to be 2062 mg mL and newline624 FU mLrespectively at 99 h The specific growth rate of the marine newlineisolate was analyzed using modified Gompertz equation with the effect of newlineultrasonication The specific growth rate 0554 h was also found to be newlinehigher with the effect of ultrasonication Optimization of parameters such as newlinepH 510 temperature 2050C and salinity 210 wv were done and newlinethe results showed that pH 7 temperature 35C and salinity 6 wv as newlineoptimum for maximum cell concentration and thrombinase activity newline newline
Pagination: xvii,174p.
Appears in Departments:Faculty of Science and Humanities

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01_title.pdfAttached File171.11 kBAdobe PDFView/Open
02_certificate.pdf2.03 MBAdobe PDFView/Open
03_abstract.pdf86 kBAdobe PDFView/Open
04_acknowledgement.pdf56.55 kBAdobe PDFView/Open
05_contents.pdf127.32 kBAdobe PDFView/Open
06_chapter 1.pdf360.49 kBAdobe PDFView/Open
07_chapter 2.pdf397.23 kBAdobe PDFView/Open
08_chapter 3.pdf177.81 kBAdobe PDFView/Open
09_chapter 4.pdf4.34 MBAdobe PDFView/Open
10_chapter 5.pdf92.14 kBAdobe PDFView/Open
11_references.pdf209.05 kBAdobe PDFView/Open
12-publications.pdf80.82 kBAdobe PDFView/Open
13_vitae.pdf53.69 kBAdobe PDFView/Open

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