Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/2383
Title: Studies on biological significance of g quadruplex DNA
Researcher: Halder, Kangkan
Guide(s): Chowdhury, Shantanu
Keywords: Biotechnology
Upload Date: 25-Aug-2011
University: University of Pune
Completed Date: March 2008
Abstract: DNA double helix is known to have conformational polymorphism such as A, B and Z forms. Apart from these, it has been found that Guanine-rich DNA sequences can adopt unusual foldback four-stranded DNA structures called ‘G-quadruplex’. A Gquadruplex structure is formed by two or more consecutive stacks of ‘G-tetrads’, which are the coplanar association of four guanines in a cyclic reverse Hoogsteen hydrogen bonding fashion. The consecutive stacks of G-tetrads in G-quadruplex structure are further stabilized by monovalent cations, especially potassium ions. The guanine-rich DNA sequences which can potentially form G-quadruplex motifs have been identified and characterized by different biophysical and biochemical methods to be present in the upstream promoter regions of proto-oncogenes such as c-MYC, c-JUN, c-KIT, c-MYB, c-REL, etc. and interestingly the 3’-telomeric sequence of many eukaryotes. It has been proposed that these structures in telomere ends play significant roles in chromosome end protection and stabilization. There are also enough circumstantial evidences for other biological roles played by these higher DNA corformations like, in replication, transcription regulation and recombination. Additional evidence of their existence and biological relevance comes from fact that these motifs are recognized by different classes of proteins, including transcription factors, nucleases, helicases, reverse transcriptases, etc. In line, G-quadruplex specific small molecules have also gained more attention than ever due to their therapeutic value as anticancer drugs for telomerase inhibition resulting from binding to G-quadruplex motifs within the human telomeres. Similar avenues have been explored by developing molecules which perturbed c-MYC expression by stabilizing the G-quadruplex motif present in c-MYC promoter region. Nevertheless, these small molecules are also bolstering the existence of G-quadruplex motif in-vivo.
Pagination: 103p.
URI: http://hdl.handle.net/10603/2383
Appears in Departments:Department of Biotechnology

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04_acknowledgement.pdf26.46 kBAdobe PDFView/Open
05_table of contents.pdf37.56 kBAdobe PDFView/Open
06_list of figures.pdf71.46 kBAdobe PDFView/Open
07_list of tables.pdf40.27 kBAdobe PDFView/Open
08_abstract.pdf36.22 kBAdobe PDFView/Open
09_abbreviations.pdf21.91 kBAdobe PDFView/Open
10_chapter1.pdf778.66 kBAdobe PDFView/Open
11_chapter2.pdf1 MBAdobe PDFView/Open
12_chapter3.pdf1.12 MBAdobe PDFView/Open
13_chapter4.pdf788.78 kBAdobe PDFView/Open
14_chapter5.pdf809.73 kBAdobe PDFView/Open
15_bibliography.pdf747.39 kBAdobe PDFView/Open
16_list of publication.pdf34.26 kBAdobe PDFView/Open


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