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Title: Studies on Molecular Phylogeny of Selected Pulmonates Mollusca_ Gastropoda and Spectroscopic Studies on the Hemocyanins of Pila Virens _Lamarck 1822_ and Pila Globosa _Swainson 1822
Researcher: Vijaya Sai Ayyagari
Guide(s): Krupanidhi Sreerama
University: Vignans Foundation for Science Technology and Research
Completed Date: 2017
Abstract: Gastropoda belonging to the second largest invertebrate phylum Mollusca is the speciose class harbouring species that exhibit great diversity in their morphology and habitats they thrive in. This class is divided into six different groups viz. Patellogastropoda, Vetigastropoda, Cocculiniformia, Neritimorpha, Caenogastropoda and Heterobranchia. Of these, Heterobranchia is divided into Lower Heterobranchia , Opisthobranchia and Pulmonata . Opisthobranchia and Pulmonata are united as Euthyneura on the basis of a diagnostic character of the nervous system (Pentaganglionate condition). Further, Euthyneura and Lower Heterobranchia (=Allogastropoda) (Triganglionate condition) are united as Heterobranchia . Among these, pulmonates are a specialized group of organisms which have developed lungs for breathing (specialization of mantle cavity). In most of the phylogenetic studies, Pulmonata is poorly represented. Therefore, in the present study, considerable attempt has been made to increase the number of pulmonate taxa representing major groups in Pulmonata by retrieving the nucleotide sequences from international nucleotide sequence databases alongside the sequences obtained in the present study. In addition, the concepts of Heterobranchia and Euthyneura (at large) as proposed on the basis of morphological and anatomical characteristics were also verified by the outcomes based on molecular phylogenetic analyses. newlineIn the present study, taxa from Pulmonata along with two out group taxa from Caenogastropoda were sampled. Phylogenetic relationships among different groups of Pulmonata (in particular) and Heterobranchia (at large) were evaluated by means of information derived from 16S, 18S, 28S, 12S rDNA, NADH6, tRNA-Proline and H3 genes. Phylogenetic analyses by concatenated dataset of 16S, 18S and 28S yielded resolved topologies with Heterobranchia and Euthyneura recovered as monophyletic and paraphyletic respectively. newline
Pagination: 313
Appears in Departments:Department of Biotechnology

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